acot14 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001030110 · chr4_KZ115093v1_alt

114 Specific

FWD 5' CCTCAGACCCAGCTCTCAAA 3'

REV 5' CAGCAGGTGTCGATTTCCTG 3'

Fwd Tm 61.0°C

Rev Tm 60.6°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001030110 · chr4

114 Specific

FWD 5' CCTCAGACCCAGCTCTCAAA 3'

REV 5' CAGCAGGTGTCGATTTCCTG 3'

Fwd Tm 61.0°C

Rev Tm 60.6°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001030110 · chr4_KZ115093v1_alt

113 Specific

FWD 5' TTCCCTCAGACCCAGCTCTC 3'

REV 5' GCAGGTGTCGATTTCCTGCT 3'

Fwd Tm 62.9°C

Rev Tm 62.4°C

GC 60%

Product 150 bp

Spans 2 introns

NM_001030110 · chr4

113 Specific

FWD 5' TTCCCTCAGACCCAGCTCTC 3'

REV 5' GCAGGTGTCGATTTCCTGCT 3'

Fwd Tm 62.9°C

Rev Tm 62.4°C

GC 60%

Product 150 bp

Spans 2 introns

NM_001030110 · chr4_KZ115093v1_alt

113 Specific

FWD 5' CCTCAGACCCAGCTCTCAAA 3'

REV 5' GCAGGTGTCGATTTCCTGCT 3'

Fwd Tm 61.0°C

Rev Tm 62.4°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001030110 · chr4_KZ115093v1_alt

113 Specific

FWD 5' CCTCAGACCCAGCTCTCAAA 3'

REV 5' AGCAGGTGTCGATTTCCTGC 3'

Fwd Tm 61.0°C

Rev Tm 62.4°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001030110 · chr4

113 Specific

FWD 5' CCTCAGACCCAGCTCTCAAA 3'

REV 5' GCAGGTGTCGATTTCCTGCT 3'

Fwd Tm 61.0°C

Rev Tm 62.4°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001030110 · chr4

113 Specific

FWD 5' CCTCAGACCCAGCTCTCAAA 3'

REV 5' AGCAGGTGTCGATTTCCTGC 3'

Fwd Tm 61.0°C

Rev Tm 62.4°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001030110 · chr4_KZ115093v1_alt

112 Specific

FWD 5' CCTCAGACCCAGCTCTCAAA 3'

REV 5' TCAGCAGGTGTCGATTTCCT 3'

Fwd Tm 61.0°C

Rev Tm 60.8°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001030110 · chr4

112 Specific

FWD 5' CCTCAGACCCAGCTCTCAAA 3'

REV 5' TCAGCAGGTGTCGATTTCCT 3'

Fwd Tm 61.0°C

Rev Tm 60.8°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001030110 · chr4_KZ115093v1_alt

112 Specific

FWD 5' TGACTGCCTACTCCAGAGTC 3'

REV 5' GGTTGTCCTCATTCACTGGC 3'

Fwd Tm 60.1°C

Rev Tm 60.6°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001030110 · chr4_KZ115093v1_alt

112 Specific

FWD 5' GACTGCCTACTCCAGAGTCA 3'

REV 5' GGTTGTCCTCATTCACTGGC 3'

Fwd Tm 60.1°C

Rev Tm 60.6°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001030110 · chr4

112 Specific

FWD 5' TGACTGCCTACTCCAGAGTC 3'

REV 5' GGTTGTCCTCATTCACTGGC 3'

Fwd Tm 60.1°C

Rev Tm 60.6°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001030110 · chr4

112 Specific

FWD 5' GACTGCCTACTCCAGAGTCA 3'

REV 5' GGTTGTCCTCATTCACTGGC 3'

Fwd Tm 60.1°C

Rev Tm 60.6°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001030110 · chr4_KZ115093v1_alt

112 Specific

FWD 5' CCCTCAGACCCAGCTCTCAA 3'

REV 5' GCAGGTGTCGATTTCCTGCT 3'

Fwd Tm 63.2°C

Rev Tm 62.4°C

GC 60%

Product 148 bp

Spans 2 introns

NM_001030110 · chr4_KZ115093v1_alt

112 Specific

FWD 5' CCCTCAGACCCAGCTCTCAA 3'

REV 5' AGCAGGTGTCGATTTCCTGC 3'

Fwd Tm 63.2°C

Rev Tm 62.4°C

GC 60%

Product 149 bp

Spans 2 introns

NM_001030110 · chr4

112 Specific

FWD 5' CCCTCAGACCCAGCTCTCAA 3'

REV 5' GCAGGTGTCGATTTCCTGCT 3'

Fwd Tm 63.2°C

Rev Tm 62.4°C

GC 60%

Product 148 bp

Spans 2 introns

NM_001030110 · chr4

112 Specific

FWD 5' CCCTCAGACCCAGCTCTCAA 3'

REV 5' AGCAGGTGTCGATTTCCTGC 3'

Fwd Tm 63.2°C

Rev Tm 62.4°C

GC 60%

Product 149 bp

Spans 2 introns

NM_001030110 · chr4_KZ115093v1_alt

110 Specific

FWD 5' TCAGACCCAGCTCTCAAAGT 3'

REV 5' GTCAGCAGGTGTCGATTTCC 3'

Fwd Tm 60.2°C

Rev Tm 60.5°C

GC 50%

Product 149 bp

Spans 2 introns

NM_001030110 · chr4

110 Specific

FWD 5' TCAGACCCAGCTCTCAAAGT 3'

REV 5' GTCAGCAGGTGTCGATTTCC 3'

Fwd Tm 60.2°C

Rev Tm 60.5°C

GC 50%

Product 149 bp

Spans 2 introns