acot19 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001007419 · chr25

111 Specific

FWD 5' CGTAGAGCCCAAGTGTGTTG 3'

REV 5' GCAACAGATCCCGCCAAATT 3'

Fwd Tm 60.5°C

Rev Tm 61.0°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001007419 · chr25

110 Specific

FWD 5' TGACCCATCACAGAAGGTCA 3'

REV 5' GAGAAGGTGGCTGTTTCCAG 3'

Fwd Tm 60.2°C

Rev Tm 60.3°C

GC 50%

Product 145 bp

Spans 2 introns

NM_001007419 · chr25

110 Specific

FWD 5' TGACCCATCACAGAAGGTCA 3'

REV 5' GTGAGAAGGTGGCTGTTTCC 3'

Fwd Tm 60.2°C

Rev Tm 60.5°C

GC 50%

Product 147 bp

Spans 2 introns

NM_001007419 · chr25

110 Specific

FWD 5' TCGCACTCCTCTCTCATCCA 3'

REV 5' TCCAGAACTGCAGGAAACGG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 138 bp

Spans 1 intron

NM_001007419 · chr25

109 Specific

FWD 5' CGTAGAGCCCAAGTGTGTTG 3'

REV 5' AGCAACAGATCCCGCCAAAT 3'

Fwd Tm 60.5°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001007419 · chr25

109 Specific

FWD 5' TCGTAGAGCCCAAGTGTGTT 3'

REV 5' GCAACAGATCCCGCCAAATT 3'

Fwd Tm 60.7°C

Rev Tm 61.0°C

GC 50%

Product 150 bp

Spans 2 introns

NM_001007419 · chr25

109 Specific

FWD 5' CGGTCGCAGGTTTCGTAAGA 3'

REV 5' TGGATGAGAGAGGAGTGCGA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 110 bp

Spans 1 intron

NM_001007419 · chr25

109 Specific

FWD 5' TCAGAACTGGCCAGCTTCAG 3'

REV 5' TGTCCAGTTCCCGGATAGGA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 105 bp

Spans 1 intron

NM_001007419 · chr25

109 Specific

FWD 5' CAGAACTGGCCAGCTTCAGA 3'

REV 5' TGTCCAGTTCCCGGATAGGA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 104 bp

Spans 1 intron

NM_001007419 · chr25

109 Specific

FWD 5' ACGCTCTTCATTCCTGCAGG 3'

REV 5' GCGTTCAACAGTCCGACGTA 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 149 bp

Spans 1 intron