acot22 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001256219 · chr16

109 Specific

FWD 5' AGATTTCCTACGCCGACAGC 3'

REV 5' CAGCTGTTATGTCCGGCAGA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 116 bp

Spans 1 intron

NM_001256219 · chr16

109 Specific

FWD 5' ATGCCCACTGGTAATGGACC 3'

REV 5' CTCTCTTTGCCAGCAGAGCT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 97 bp

Spans 1 intron

NM_001256219 · chr16

109 Specific

FWD 5' GCCCACTGGTAATGGACCAT 3'

REV 5' CTCTCTTTGCCAGCAGAGCT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 95 bp

Spans 1 intron

NM_001256219 · chr16

109 Specific

FWD 5' ATGCCCACTGGTAATGGACC 3'

REV 5' TCTCTTTGCCAGCAGAGCTG 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 96 bp

Spans 1 intron

NM_001256219 · chr16

109 Specific

FWD 5' GCCCACTGGTAATGGACCAT 3'

REV 5' TCTCTTTGCCAGCAGAGCTG 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 94 bp

Spans 1 intron

NM_001256219 · chr16

109 Specific

FWD 5' AGATTTCCTACGCCGACAGC 3'

REV 5' ACAGCAGCTGTTATGTCCGG 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 120 bp

Spans 1 intron

NM_001256219 · chr16

109 Specific

FWD 5' AGGGTGTCCGGAGAAGTCTT 3'

REV 5' TCTCTTTGCCAGCAGAGCTG 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001256219 · chr16

108 Specific

FWD 5' AGATTTCCTACGCCGACAGC 3'

REV 5' CCGTTGATCCAAACAGCAGC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 132 bp

Spans 1 intron

NM_001256219 · chr16

108 Specific

FWD 5' GACAGCCAGAGGTGAAAGGT 3'

REV 5' GGCATTGCATCCGTTGATCC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 128 bp

Spans 1 intron

NM_001256219 · chr16

108 Specific

FWD 5' ACAGCCAGAGGTGAAAGGTC 3'

REV 5' GGCATTGCATCCGTTGATCC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 127 bp

Spans 1 intron