acp7 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001099250 · chrUn_KN150131v1

119 Specific

FWD 5' TACGGAGTGGATCTGGAGCT 3'

REV 5' CCAGCTGAACCGGTGATGAT 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001099250 · chrUn_KN150131v1

119 Specific

FWD 5' TACGGAGTGGATCTGGAGCT 3'

REV 5' ATCCAGCTGAACCGGTGATG 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001099250 · chrUn_KN150131v1

119 Specific

FWD 5' TACGGAGTGGATCTGGAGCT 3'

REV 5' CATCCAGCTGAACCGGTGAT 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001099250 · chrUn_KN150131v1

119 Specific

FWD 5' CAGATCGGCACGTATGACGT 3'

REV 5' GACAGGTCATGTACGGCACA 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001099250 · chrUn_KN150131v1

119 Specific

FWD 5' AAGCTATGTGCGACTCGGAC 3'

REV 5' GTGTTCATGAGCCCAGAGCT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 106 bp

Spans 2 introns

NM_001099250 · chrUn_KN150131v1

119 Specific

FWD 5' GATCGGCACGTATGACGTCA 3'

REV 5' GACAGGTCATGTACGGCACA 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001099250 · chrUn_KN150131v1

119 Specific

FWD 5' TGAGAGACTCTGGCCTGTGT 3'

REV 5' AAGCCGTCGTGTTTCTCTCG 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 129 bp

Spans 2 introns

NM_001099250 · chrUn_KN150131v1

119 Specific

FWD 5' TGACGACTGCACTCACTTCC 3'

REV 5' TGTTCATGAGCCCAGAGCTC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 126 bp

Spans 2 introns

NM_001099250 · chrUn_KN150131v1

119 Specific

FWD 5' GACGACTGCACTCACTTCCA 3'

REV 5' TGTTCATGAGCCCAGAGCTC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 125 bp

Spans 2 introns

NM_001099250 · chrUn_KN150131v1

118 Specific

FWD 5' ACGTCATTCTGCACATCGGG 3'

REV 5' GTACGGCACATAAGCAGCGA 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 110 bp

Spans 2 introns