acta1a 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001017750 · chr1

110 Specific

FWD 5' CGCTCTTCCTCATGCCATCA 3'

REV 5' CACGGACAATCTCACGCTCA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 125 bp

Spans 1 intron

NM_001017750 · chr1

110 Specific

FWD 5' CGCTCTTCCTCATGCCATCA 3'

REV 5' GTCACGGACAATCTCACGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 127 bp

Spans 1 intron

NM_001017750 · chr1

110 Specific

FWD 5' ACGCTCTTCCTCATGCCATC 3'

REV 5' CACGGACAATCTCACGCTCA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001017750 · chr1

110 Specific

FWD 5' ACGCTCTTCCTCATGCCATC 3'

REV 5' GTCACGGACAATCTCACGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 128 bp

Spans 1 intron

NM_001017750 · chr1

110 Specific

FWD 5' CATGCGTTTGGACTTGGCTG 3'

REV 5' CACGGACAATCTCACGCTCA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 107 bp

Spans 1 intron

NM_001017750 · chr1

110 Specific

FWD 5' CATGCGTTTGGACTTGGCTG 3'

REV 5' GTCACGGACAATCTCACGCT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 109 bp

Spans 1 intron

NM_001017750 · chr1

110 Specific

FWD 5' CATGCGTTTGGACTTGGCTG 3'

REV 5' TCCAGGGCAACATAGCACAG 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001017750 · chr1

110 Specific

FWD 5' CATGCGTTTGGACTTGGCTG 3'

REV 5' AGTCCAGGGCAACATAGCAC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 143 bp

Spans 1 intron

NM_001017750 · chr1

109 Specific

FWD 5' CCCGCTATGTATGTGGCCAT 3'

REV 5' TGATGGCATGAGGAAGAGCG 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 139 bp

Spans 1 intron

NM_001017750 · chr1

109 Specific

FWD 5' CCCGCTATGTATGTGGCCAT 3'

REV 5' GATGGCATGAGGAAGAGCGT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 138 bp

Spans 1 intron