actc1a 8 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001001409 · chr20

110 Specific

FWD 5' AGGAAATCACTGCTCTGGCC 3'

REV 5' TGCTGATCCACATCTGCTGG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 132 bp

Spans 1 intron

NM_001001409 · chr20

110 Specific

FWD 5' CTGGATTCGCCGGTGATGAT 3'

REV 5' TGAGCCTCGTCTCCCACATA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 121 bp

Spans 1 intron

NM_001001409 · chr20

109 Specific

FWD 5' ACTCTTTCGTCACCACTGCC 3'

REV 5' ACCATCAGGCAACTCGTAGC 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 143 bp

Spans 1 intron

NM_001001409 · chr20

109 Specific

FWD 5' TCTGGTTTGGTCAAGGCTGG 3'

REV 5' TGAGCCTCGTCTCCCACATA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 137 bp

Spans 1 intron

NM_001001409 · chr20

109 Specific

FWD 5' CTGGTTTGGTCAAGGCTGGA 3'

REV 5' TGAGCCTCGTCTCCCACATA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 136 bp

Spans 1 intron

NM_001001409 · chr20

109 Specific

FWD 5' ACGAGTTGCCTGATGGTCAG 3'

REV 5' GCAGTTTCATGGATGCCAGC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 115 bp

Spans 1 intron

NM_001001409 · chr20

109 Specific

FWD 5' GCTACGAGTTGCCTGATGGT 3'

REV 5' GCAGTTTCATGGATGCCAGC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001001409 · chr20

109 Specific

FWD 5' ACTCTTTCGTCACCACTGCC 3'

REV 5' CTGACCATCAGGCAACTCGT 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 146 bp

Spans 1 intron