actc2 9 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001002066 · chr20_KZ114968v1_alt

109 Specific

FWD 5' CCGGCCATGTATGTGGCTAT 3'

REV 5' TGGGTCACTCCATCTCCAGA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 95 bp

Spans 1 intron

NM_001002066 · chr20_KZ114968v1_alt

109 Specific

FWD 5' CGTGCTGTCTTTCCCTCCAT 3'

REV 5' CCGTGCTCAATCGGGTACTT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 140 bp

Spans 1 intron

NM_001002066 · chr20_KZ114968v1_alt

109 Specific

FWD 5' GATGCTCCTCGTGCTGTCTT 3'

REV 5' CCGTGCTCAATCGGGTACTT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001002066 · chr20_KZ114968v1_alt

109 Specific

FWD 5' TGCTCCTCGTGCTGTCTTTC 3'

REV 5' CCGTGCTCAATCGGGTACTT 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001002066 · chr20_KZ114968v1_alt

109 Specific

FWD 5' AACAACGTCCTATCTGGCGG 3'

REV 5' ATGGAGCCACCAATCCAGAC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001002066 · chr20_KZ114968v1_alt

109 Specific

FWD 5' TGCTGACCGTATGCAGAAGG 3'

REV 5' ATGGAGCCACCAATCCAGAC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 108 bp

Spans 1 intron

NM_001002066 · chr20_KZ114968v1_alt

109 Specific

FWD 5' TCCATTGTTGGTCGTCCTCG 3'

REV 5' CCGTGCTCAATCGGGTACTT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 125 bp

Spans 1 intron

NM_001002066 · chr20_KZ114968v1_alt

109 Specific

FWD 5' TGTTCCGGCCATGTATGTGG 3'

REV 5' TCCATCTCCAGAGTCCAGCA 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 91 bp

Spans 1 intron

NM_001002066 · chr20_KZ114968v1_alt

109 Specific

FWD 5' ATGGCTACTGCTGCTTCCTC 3'

REV 5' TCATGGATGCCAGCAGACTC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 149 bp

Spans 1 intron