actl6b 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001020740 · chr23

120 Specific

FWD 5' ACGGCATGATTGAGGACTGG 3'

REV 5' ACGTACCCATCATGCACAGG 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 142 bp

Spans 3 introns

NM_001020740 · chr23

120 Specific

FWD 5' TACGATGAACAGGTGGCTGC 3'

REV 5' CAGCATGGTGTTACCGGACA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001020740 · chr23

120 Specific

FWD 5' ACGGCATGATTGAGGACTGG 3'

REV 5' GGACGTACCCATCATGCACA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 144 bp

Spans 3 introns

NM_001020740 · chr23

119 Specific

FWD 5' GATGAACAGGTGGCTGCTCA 3'

REV 5' CAGCATGGTGTTACCGGACA 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001020740 · chr23

119 Specific

FWD 5' ACGGCATGATTGAGGACTGG 3'

REV 5' ACCCATCATGCACAGGGATG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 138 bp

Spans 3 introns

NM_001020740 · chr23

119 Specific

FWD 5' ATGAACAGGTGGCTGCTCAG 3'

REV 5' CAGCATGGTGTTACCGGACA 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001020740 · chr23

119 Specific

FWD 5' AACAGGTGGCTGCTCAGATG 3'

REV 5' CAGCATGGTGTTACCGGACA 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001020740 · chr23

119 Specific

FWD 5' ACCGAGCATGAGACTGAAGC 3'

REV 5' TCCGCCCTCATCATACTCCT 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001020740 · chr23

119 Specific

FWD 5' GAACAGGTGGCTGCTCAGAT 3'

REV 5' CAGCATGGTGTTACCGGACA 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001020740 · chr23

118 Specific

FWD 5' ACGGCATGATTGAGGACTGG 3'

REV 5' AGGACGTACCCATCATGCAC 3'

Fwd Tm 62.0°C

Rev Tm 61.7°C

GC 55%

Product 145 bp

Spans 3 introns