acy3.2 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001002347 · chr1

117 Specific

FWD 5' TTTCAATTGAGGTCGGGCCG 3'

REV 5' AGGCTTCCACATCTCCACCT 3'

Fwd Tm 62.7°C

Rev Tm 62.8°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001002347 · chr1

115 Specific

FWD 5' TTTCAATTGAGGTCGGGCCG 3'

REV 5' GGCTTCCACATCTCCACCTT 3'

Fwd Tm 62.7°C

Rev Tm 61.7°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001002347 · chr1

115 Specific

FWD 5' TTTCAATTGAGGTCGGGCCG 3'

REV 5' AAGGCTTCCACATCTCCACC 3'

Fwd Tm 62.7°C

Rev Tm 61.7°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001002347 · chr1

110 Specific

FWD 5' GGGACCGTATTTGAAGGTGG 3'

REV 5' CCCGTTTGAGGAGACAGAAG 3'

Fwd Tm 60.1°C

Rev Tm 59.7°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001002347 · chr1

109 Specific

FWD 5' ACCCACGAGCTGTGAAAGAG 3'

REV 5' CGGACTTCATATGGGCTGCT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 112 bp

Spans 1 intron

NM_001002347 · chr1

109 Specific

FWD 5' CCCACGAGCTGTGAAAGAGT 3'

REV 5' CGGACTTCATATGGGCTGCT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 111 bp

Spans 1 intron

NM_001002347 · chr1

109 Specific

FWD 5' GGTGTATGGCCGATTCCTGT 3'

REV 5' TCGGACTTCATATGGGCTGC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001002347 · chr1

109 Specific

FWD 5' ACCCACGAGCTGTGAAAGAG 3'

REV 5' TCGGACTTCATATGGGCTGC 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 113 bp

Spans 1 intron

NM_001002347 · chr1

109 Specific

FWD 5' CCCACGAGCTGTGAAAGAGT 3'

REV 5' TCGGACTTCATATGGGCTGC 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 112 bp

Spans 1 intron

NM_001002347 · chr1

109 Specific

FWD 5' GTCAAACCCACGAGCTGTGA 3'

REV 5' AATTCCTGAGCACGTCGGAC 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 132 bp

Spans 1 intron