adal 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001033744 · chr25_KZ115735v1_alt

119 Specific

FWD 5' GAGACCCAACCGTTGGTCAT 3'

REV 5' GTCAGGAGGCAGGTTCAACA 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001033744 · chr25_KZ115735v1_alt

119 Specific

FWD 5' GAGACCCAACCGTTGGTCAT 3'

REV 5' TGTCAGGAGGCAGGTTCAAC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001033744 · chr25

119 Specific

FWD 5' GAGACCCAACCGTTGGTCAT 3'

REV 5' GTCAGGAGGCAGGTTCAACA 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001033744 · chr25

119 Specific

FWD 5' GAGACCCAACCGTTGGTCAT 3'

REV 5' TGTCAGGAGGCAGGTTCAAC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001033744 · chr25_KZ115735v1_alt

119 Specific

FWD 5' AAGTGGAGACCCAACCGTTG 3'

REV 5' GTCAGGAGGCAGGTTCAACA 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001033744 · chr25

119 Specific

FWD 5' AAGTGGAGACCCAACCGTTG 3'

REV 5' GTCAGGAGGCAGGTTCAACA 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001033744 · chr25_KZ115735v1_alt

118 Specific

FWD 5' AGACCCAACCGTTGGTCATG 3'

REV 5' GTCAGGAGGCAGGTTCAACA 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001033744 · chr25_KZ115735v1_alt

118 Specific

FWD 5' AGACCCAACCGTTGGTCATG 3'

REV 5' TGTCAGGAGGCAGGTTCAAC 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001033744 · chr25

118 Specific

FWD 5' AGACCCAACCGTTGGTCATG 3'

REV 5' GTCAGGAGGCAGGTTCAACA 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001033744 · chr25

118 Specific

FWD 5' AGACCCAACCGTTGGTCATG 3'

REV 5' TGTCAGGAGGCAGGTTCAAC 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001033744 · chr25_KZ115735v1_alt

118 Specific

FWD 5' GAGACCCAACCGTTGGTCAT 3'

REV 5' CTGTCAGGAGGCAGGTTCAA 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001033744 · chr25

118 Specific

FWD 5' GAGACCCAACCGTTGGTCAT 3'

REV 5' CTGTCAGGAGGCAGGTTCAA 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001033744 · chr25_KZ115735v1_alt

118 Specific

FWD 5' CGTAGCACACCACGAGAAGT 3'

REV 5' CGGTCTACAGCCACCAGAAA 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001033744 · chr25

118 Specific

FWD 5' CGTAGCACACCACGAGAAGT 3'

REV 5' CGGTCTACAGCCACCAGAAA 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001033744 · chr25_KZ115735v1_alt

118 Specific

FWD 5' AGCACACCACGAGAAGTGAC 3'

REV 5' CGGTCTACAGCCACCAGAAA 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 55%

Product 131 bp

Spans 2 introns

NM_001033744 · chr25_KZ115735v1_alt

118 Specific

FWD 5' GCACACCACGAGAAGTGACT 3'

REV 5' CGGTCTACAGCCACCAGAAA 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 55%

Product 130 bp

Spans 2 introns

NM_001033744 · chr25

118 Specific

FWD 5' AGCACACCACGAGAAGTGAC 3'

REV 5' CGGTCTACAGCCACCAGAAA 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 55%

Product 131 bp

Spans 2 introns

NM_001033744 · chr25

118 Specific

FWD 5' GCACACCACGAGAAGTGACT 3'

REV 5' CGGTCTACAGCCACCAGAAA 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 55%

Product 130 bp

Spans 2 introns

NM_001033744 · chr25_KZ115735v1_alt

118 Specific

FWD 5' AAGTGGAGACCCAACCGTTG 3'

REV 5' CTGTCAGGAGGCAGGTTCAA 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001033744 · chr25

118 Specific

FWD 5' AAGTGGAGACCCAACCGTTG 3'

REV 5' CTGTCAGGAGGCAGGTTCAA 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 150 bp

Spans 2 introns