add1 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001079958 · chr21

119 Specific

FWD 5' AAGACGAAGGTCAACCGCTG 3'

REV 5' TCTTAAAGTAGCGGCGTCGC 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001079958 · chr21

119 Specific

FWD 5' AAGACGAAGGTCAACCGCTG 3'

REV 5' CTTAAAGTAGCGGCGTCGCT 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001079958 · chr21

119 Specific

FWD 5' TGTCCGGCTGAACTCTGAAC 3'

REV 5' CACCCAGATTAGTGCTGCCT 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001079958 · chr21

119 Specific

FWD 5' AGAAGACGAAGGTCAACCGC 3'

REV 5' TCTTAAAGTAGCGGCGTCGC 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001079958 · chr21

119 Specific

FWD 5' GAAGACGAAGGTCAACCGCT 3'

REV 5' TCTTAAAGTAGCGGCGTCGC 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001079958 · chr21

119 Specific

FWD 5' TGTCCGGCTGAACTCTGAAC 3'

REV 5' GCTGCCTCTGTCCACAATCT 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 124 bp

Spans 2 introns

NM_001079958 · chr21

119 Specific

FWD 5' TGTCCGGCTGAACTCTGAAC 3'

REV 5' ACACCCAGATTAGTGCTGCC 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001079958 · chr21

119 Specific

FWD 5' AGAAGACGAAGGTCAACCGC 3'

REV 5' CTTAAAGTAGCGGCGTCGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001079958 · chr21

119 Specific

FWD 5' GAAGACGAAGGTCAACCGCT 3'

REV 5' CTTAAAGTAGCGGCGTCGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001079958 · chr21

119 Specific

FWD 5' GATGCGCAACAAGATCCGTG 3'

REV 5' CTTTGGATGCGGTCACAAGC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 128 bp

Spans 2 introns