adh5 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_131849 · chr14

110 Specific

FWD 5' TTGGTGGCTGGAAGAGTGTG 3'

REV 5' TCTTCCCAGCATGCATCAGG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 1 intron

NM_131849 · chr14_KZ115467v1_alt

110 Specific

FWD 5' TTGGTGGCTGGAAGAGTGTG 3'

REV 5' TCTTCCCAGCATGCATCAGG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 1 intron

NM_131849 · chr14

109 Specific

FWD 5' TTGGTGGCTGGAAGAGTGTG 3'

REV 5' AAGGCCTCATTGATCTGGGC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 121 bp

Spans 1 intron

NM_131849 · chr14_KZ115467v1_alt

109 Specific

FWD 5' TTGGTGGCTGGAAGAGTGTG 3'

REV 5' AAGGCCTCATTGATCTGGGC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 121 bp

Spans 1 intron

NM_131849 · chr14

109 Specific

FWD 5' GCCTTTGACCTGATGCATGC 3'

REV 5' GTTTGTTTGCAGGACGAGGC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 93 bp

Spans 1 intron

NM_131849 · chr14_KZ115467v1_alt

109 Specific

FWD 5' GCCTTTGACCTGATGCATGC 3'

REV 5' GTTTGTTTGCAGGACGAGGC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 93 bp

Spans 1 intron

NM_131849 · chr14

109 Specific

FWD 5' AAGGTTGAAGCGGGCTCTAC 3'

REV 5' TGACGTCGATGCCGATGATC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 112 bp

Spans 1 intron

NM_131849 · chr14_KZ115467v1_alt

109 Specific

FWD 5' AAGGTTGAAGCGGGCTCTAC 3'

REV 5' TGACGTCGATGCCGATGATC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 112 bp

Spans 1 intron

NM_131849 · chr14

109 Specific

FWD 5' ACTGCTAAGGTTGAAGCGGG 3'

REV 5' AACTTGTCCGGGTTGACGTC 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 131 bp

Spans 1 intron

NM_131849 · chr14_KZ115467v1_alt

109 Specific

FWD 5' ACTGCTAAGGTTGAAGCGGG 3'

REV 5' AACTTGTCCGGGTTGACGTC 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 131 bp

Spans 1 intron

NM_131849 · chr14

109 Specific

FWD 5' TTGGTGGCTGGAAGAGTGTG 3'

REV 5' GACGAGGCTCAGGCTTCAAT 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 197 bp

Spans 2 introns

NM_131849 · chr14_KZ115467v1_alt

109 Specific

FWD 5' TTGGTGGCTGGAAGAGTGTG 3'

REV 5' GACGAGGCTCAGGCTTCAAT 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 197 bp

Spans 2 introns

NM_131849 · chr14

109 Specific

FWD 5' TGGAGCTGACAGATGGAGGA 3'

REV 5' GCCGGGTGGAGATTTCTTGT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 150 bp

Spans 1 intron

NM_131849 · chr14_KZ115467v1_alt

109 Specific

FWD 5' TGGAGCTGACAGATGGAGGA 3'

REV 5' GCCGGGTGGAGATTTCTTGT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 150 bp

Spans 1 intron

NM_131849 · chr14

109 Specific

FWD 5' CTCCACCGAAAGCCCATGAA 3'

REV 5' TGACAGGAAACAAGCCCTCG 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 108 bp

Spans 1 intron

NM_131849 · chr14

109 Specific

FWD 5' TCCACCGAAAGCCCATGAAG 3'

REV 5' TGACAGGAAACAAGCCCTCG 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 107 bp

Spans 1 intron

NM_131849 · chr14

109 Specific

FWD 5' CGAGGGCTTGTTTCCTGTCA 3'

REV 5' TACAGAACTTGCACTCGCCG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 140 bp

Spans 1 intron

NM_131849 · chr14_KZ115467v1_alt

109 Specific

FWD 5' CTCCACCGAAAGCCCATGAA 3'

REV 5' TGACAGGAAACAAGCCCTCG 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 108 bp

Spans 1 intron

NM_131849 · chr14_KZ115467v1_alt

109 Specific

FWD 5' TCCACCGAAAGCCCATGAAG 3'

REV 5' TGACAGGAAACAAGCCCTCG 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 107 bp

Spans 1 intron

NM_131849 · chr14_KZ115467v1_alt

109 Specific

FWD 5' CGAGGGCTTGTTTCCTGTCA 3'

REV 5' TACAGAACTTGCACTCGCCG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 140 bp

Spans 1 intron