adh8b 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_203460 · chr13

110 Specific

FWD 5' TTGACCTTCCTGCGTCCACA 3'

REV 5' CAGCGGCCCGACATTTGATC 3'

Fwd Tm 63.6°C

Rev Tm 63.7°C

GC 55%

Product 90 bp

Spans 2 introns

NM_203460 · chr13_KZ115407v1_alt

110 Specific

FWD 5' TTGACCTTCCTGCGTCCACA 3'

REV 5' CAGCGGCCCGACATTTGATC 3'

Fwd Tm 63.6°C

Rev Tm 63.7°C

GC 55%

Product 90 bp

Spans 2 introns

NM_203460 · chr13

109 Specific

FWD 5' GTTGTTTGCATACCGCGCAC 3'

REV 5' CAGCGGCCCGACATTTGATC 3'

Fwd Tm 62.6°C

Rev Tm 63.7°C

GC 55%

Product 144 bp

Spans 2 introns

NM_203460 · chr13_KZ115407v1_alt

109 Specific

FWD 5' GTTGTTTGCATACCGCGCAC 3'

REV 5' CAGCGGCCCGACATTTGATC 3'

Fwd Tm 62.6°C

Rev Tm 63.7°C

GC 55%

Product 144 bp

Spans 2 introns

NM_203460 · chr13

109 Specific

FWD 5' TAGAGAGCGTTGGACCTGGA 3'

REV 5' GCTGCGCTCACACAGATTTG 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 125 bp

Spans 1 intron

NM_203460 · chr13_KZ115407v1_alt

109 Specific

FWD 5' TAGAGAGCGTTGGACCTGGA 3'

REV 5' GCTGCGCTCACACAGATTTG 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 125 bp

Spans 1 intron

NM_203460 · chr13

109 Specific

FWD 5' TTTGACCTTCCTGCGTCCAC 3'

REV 5' CAGCGGCCCGACATTTGATC 3'

Fwd Tm 62.3°C

Rev Tm 63.7°C

GC 55%

Product 91 bp

Spans 2 introns

NM_203460 · chr13_KZ115407v1_alt

109 Specific

FWD 5' TTTGACCTTCCTGCGTCCAC 3'

REV 5' CAGCGGCCCGACATTTGATC 3'

Fwd Tm 62.3°C

Rev Tm 63.7°C

GC 55%

Product 91 bp

Spans 2 introns

NM_203460 · chr13

109 Specific

FWD 5' GTTGTTTGCATACCGCGCAC 3'

REV 5' AGCGGCCCGACATTTGATCA 3'

Fwd Tm 62.6°C

Rev Tm 64.3°C

GC 55%

Product 143 bp

Spans 2 introns

NM_203460 · chr13_KZ115407v1_alt

109 Specific

FWD 5' GTTGTTTGCATACCGCGCAC 3'

REV 5' AGCGGCCCGACATTTGATCA 3'

Fwd Tm 62.6°C

Rev Tm 64.3°C

GC 55%

Product 143 bp

Spans 2 introns

NM_203460 · chr13

109 Specific

FWD 5' CAAATCTGTGTGAGCGCAGC 3'

REV 5' AACTGCAGGATGGGCTTACC 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 97 bp

Spans 1 intron

NM_203460 · chr13_KZ115407v1_alt

109 Specific

FWD 5' CAAATCTGTGTGAGCGCAGC 3'

REV 5' AACTGCAGGATGGGCTTACC 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 97 bp

Spans 1 intron

NM_203460 · chr13

109 Specific

FWD 5' AGCAGCTGGAGTGGTAGAGA 3'

REV 5' GCTGCGCTCACACAGATTTG 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 139 bp

Spans 1 intron

NM_203460 · chr13_KZ115407v1_alt

109 Specific

FWD 5' AGCAGCTGGAGTGGTAGAGA 3'

REV 5' GCTGCGCTCACACAGATTTG 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 139 bp

Spans 1 intron

NM_203460 · chr13

109 Specific

FWD 5' TTTGACCTTCCTGCGTCCAC 3'

REV 5' AGCGGCCCGACATTTGATCA 3'

Fwd Tm 62.3°C

Rev Tm 64.3°C

GC 55%

Product 90 bp

Spans 2 introns

NM_203460 · chr13

109 Specific

FWD 5' CAAATCTGTGTGAGCGCAGC 3'

REV 5' GCCAGAGTTCCTGTGTTGGT 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 118 bp

Spans 1 intron

NM_203460 · chr13_KZ115407v1_alt

109 Specific

FWD 5' TTTGACCTTCCTGCGTCCAC 3'

REV 5' AGCGGCCCGACATTTGATCA 3'

Fwd Tm 62.3°C

Rev Tm 64.3°C

GC 55%

Product 90 bp

Spans 2 introns

NM_203460 · chr13_KZ115407v1_alt

109 Specific

FWD 5' CAAATCTGTGTGAGCGCAGC 3'

REV 5' GCCAGAGTTCCTGTGTTGGT 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 118 bp

Spans 1 intron

NM_203460 · chr13

109 Specific

FWD 5' TGGGCTGTGGAGTCACTACT 3'

REV 5' AAAGATGCGAGATGCTCCGG 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 146 bp

Spans 1 intron

NM_203460 · chr13_KZ115407v1_alt

109 Specific

FWD 5' TGGGCTGTGGAGTCACTACT 3'

REV 5' AAAGATGCGAGATGCTCCGG 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 146 bp

Spans 1 intron