adnpa 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001080015 · chr11_KZ115359v1_alt

108 Specific

FWD 5' TGCAAAGAGCATGTGGAGGA 3'

REV 5' CGGGCAATCTGAGCAGCAAA 3'

Fwd Tm 61.4°C

Rev Tm 63.2°C

GC 50%

Product 150 bp

Spans 2 introns

NM_001080015 · chr11_KZ115359v1_alt

108 Specific

FWD 5' GCAAAGAGCATGTGGAGGAC 3'

REV 5' CGGGCAATCTGAGCAGCAAA 3'

Fwd Tm 60.9°C

Rev Tm 63.2°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001080015 · chr11

108 Specific

FWD 5' TGCAAAGAGCATGTGGAGGA 3'

REV 5' CGGGCAATCTGAGCAGCAAA 3'

Fwd Tm 61.4°C

Rev Tm 63.2°C

GC 50%

Product 150 bp

Spans 2 introns

NM_001080015 · chr11

108 Specific

FWD 5' GCAAAGAGCATGTGGAGGAC 3'

REV 5' CGGGCAATCTGAGCAGCAAA 3'

Fwd Tm 60.9°C

Rev Tm 63.2°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001080015 · chr11_KZ115359v1_alt

108 Specific

FWD 5' AGGGAATTCCATGCGAGACC 3'

REV 5' AGGCCAATGTCACTCAGCAG 3'

Fwd Tm 61.8°C

Rev Tm 62.2°C

GC 55%

Product 122 bp

Spans 1 intron

NM_001080015 · chr11

108 Specific

FWD 5' AGGGAATTCCATGCGAGACC 3'

REV 5' AGGCCAATGTCACTCAGCAG 3'

Fwd Tm 61.8°C

Rev Tm 62.2°C

GC 55%

Product 122 bp

Spans 1 intron

NM_001080015 · chr11_KZ115359v1_alt

107 Specific

FWD 5' AGGGAATTCCATGCGAGACC 3'

REV 5' TCTTCCTGGCCTTCCTCAAC 3'

Fwd Tm 61.8°C

Rev Tm 61.4°C

GC 55%

Product 91 bp

Spans 1 intron

NM_001080015 · chr11

107 Specific

FWD 5' AGGGAATTCCATGCGAGACC 3'

REV 5' TCTTCCTGGCCTTCCTCAAC 3'

Fwd Tm 61.8°C

Rev Tm 61.4°C

GC 55%

Product 91 bp

Spans 1 intron

NM_001080015 · chr11_KZ115359v1_alt

107 Specific

FWD 5' ACTGCTGAGTGACATTGGCC 3'

REV 5' GAGGCAAACGGGCAATCTGA 3'

Fwd Tm 62.4°C

Rev Tm 62.7°C

GC 55%

Product 186 bp

Spans 2 introns

NM_001080015 · chr11

107 Specific

FWD 5' ACTGCTGAGTGACATTGGCC 3'

REV 5' GAGGCAAACGGGCAATCTGA 3'

Fwd Tm 62.4°C

Rev Tm 62.7°C

GC 55%

Product 186 bp

Spans 2 introns

NM_001080015 · chr11_KZ115359v1_alt

107 Specific

FWD 5' ACTGCTGAGTGACATTGGCC 3'

REV 5' AGGCAAACGGGCAATCTGAG 3'

Fwd Tm 62.4°C

Rev Tm 62.7°C

GC 55%

Product 185 bp

Spans 2 introns

NM_001080015 · chr11

107 Specific

FWD 5' ACTGCTGAGTGACATTGGCC 3'

REV 5' AGGCAAACGGGCAATCTGAG 3'

Fwd Tm 62.4°C

Rev Tm 62.7°C

GC 55%

Product 185 bp

Spans 2 introns

NM_001080015 · chr11_KZ115359v1_alt

107 Specific

FWD 5' AGGGAATTCCATGCGAGACC 3'

REV 5' GGCCAATGTCACTCAGCAGT 3'

Fwd Tm 61.8°C

Rev Tm 62.4°C

GC 55%

Product 121 bp

Spans 1 intron

NM_001080015 · chr11

107 Specific

FWD 5' AGGGAATTCCATGCGAGACC 3'

REV 5' GGCCAATGTCACTCAGCAGT 3'

Fwd Tm 61.8°C

Rev Tm 62.4°C

GC 55%

Product 121 bp

Spans 1 intron

NM_001080015 · chr11_KZ115359v1_alt

107 Specific

FWD 5' AGAGCATGTGGAGGACTACA 3'

REV 5' CAAACGGGCAATCTGAGCAG 3'

Fwd Tm 59.7°C

Rev Tm 61.4°C

GC 50%

Product 149 bp

Spans 2 introns

NM_001080015 · chr11

107 Specific

FWD 5' AGAGCATGTGGAGGACTACA 3'

REV 5' CAAACGGGCAATCTGAGCAG 3'

Fwd Tm 59.7°C

Rev Tm 61.4°C

GC 50%

Product 149 bp

Spans 2 introns

NM_001080015 · chr11_KZ115359v1_alt

107 Specific

FWD 5' CTGCTGAGTGACATTGGCCT 3'

REV 5' GAGGCAAACGGGCAATCTGA 3'

Fwd Tm 62.2°C

Rev Tm 62.7°C

GC 55%

Product 185 bp

Spans 2 introns

NM_001080015 · chr11

107 Specific

FWD 5' CTGCTGAGTGACATTGGCCT 3'

REV 5' GAGGCAAACGGGCAATCTGA 3'

Fwd Tm 62.2°C

Rev Tm 62.7°C

GC 55%

Product 185 bp

Spans 2 introns

NM_001080015 · chr11_KZ115359v1_alt

107 Specific

FWD 5' CATGCGAGACCGCGAAGAAA 3'

REV 5' GGCCAATGTCACTCAGCAGT 3'

Fwd Tm 62.8°C

Rev Tm 62.4°C

GC 55%

Product 112 bp

Spans 1 intron

NM_001080015 · chr11

107 Specific

FWD 5' CATGCGAGACCGCGAAGAAA 3'

REV 5' GGCCAATGTCACTCAGCAGT 3'

Fwd Tm 62.8°C

Rev Tm 62.4°C

GC 55%

Product 112 bp

Spans 1 intron