afap1l1b 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001077287 · chr21

117 Specific

FWD 5' TGACTGTGACCTGCAGGATC 3'

REV 5' AAGAGGAGGAGGAGGAGTGT 3'

Fwd Tm 61.3°C

Rev Tm 61.3°C

GC 55%

Product 121 bp

Spans 2 introns

NM_001077287 · chr21

117 Specific

FWD 5' CGAGAGCTGGCTAAAGGTCA 3'

REV 5' GCGCTGTCAGAATCAGAGGT 3'

Fwd Tm 61.3°C

Rev Tm 61.8°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001077287 · chr21

116 Specific

FWD 5' ACGTGGTCAGAAACCTGGTC 3'

REV 5' CCTGAGATCCTGCAGGTCAC 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001077287 · chr21

116 Specific

FWD 5' CGTGGTCAGAAACCTGGTCA 3'

REV 5' CCTGAGATCCTGCAGGTCAC 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001077287 · chr21

116 Specific

FWD 5' GACTGTGACCTGCAGGATCT 3'

REV 5' AAGAGGAGGAGGAGGAGTGT 3'

Fwd Tm 61.0°C

Rev Tm 61.3°C

GC 55%

Product 120 bp

Spans 2 introns

NM_001077287 · chr21

116 Specific

FWD 5' ACTGCGGAAACTAGAGCTGG 3'

REV 5' ACGTCCCTTTCTTGGCCTTT 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 125 bp

Spans 2 introns

NM_001077287 · chr21

116 Specific

FWD 5' ACTGCGGAAACTAGAGCTGG 3'

REV 5' AACGTCCCTTTCTTGGCCTT 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 126 bp

Spans 2 introns

NM_001077287 · chr21

116 Specific

FWD 5' ACTGCGGAAACTAGAGCTGG 3'

REV 5' AAACGTCCCTTTCTTGGCCT 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 127 bp

Spans 2 introns

NM_001077287 · chr21

116 Specific

FWD 5' CGTGGTCAGAAACCTGGTCA 3'

REV 5' GAGATCCTGCAGGTCACAGT 3'

Fwd Tm 61.7°C

Rev Tm 61.0°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001077287 · chr21

116 Specific

FWD 5' CGTGGTCAGAAACCTGGTCA 3'

REV 5' TGAGATCCTGCAGGTCACAG 3'

Fwd Tm 61.7°C

Rev Tm 61.0°C

GC 55%

Product 143 bp

Spans 2 introns