afmid 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001017677 · chr11

119 Specific

FWD 5' GGTTACAGCATCGCTCCGAA 3'

REV 5' GCTCCTGCTGAATGACCACA 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 128 bp

Spans 2 introns

NM_001017677 · chr11

119 Specific

FWD 5' TACAGCATCGCTCCGAAAGG 3'

REV 5' AGCTCCTGCTGAATGACCAC 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 126 bp

Spans 2 introns

NM_001017677 · chr11

119 Specific

FWD 5' GGTTACAGCATCGCTCCGAA 3'

REV 5' AGCTCCTGCTGAATGACCAC 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 129 bp

Spans 2 introns

NM_001017677 · chr11

119 Specific

FWD 5' TACAGCATCGCTCCGAAAGG 3'

REV 5' ACCATGGCTGCTAGATGAGC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001017677 · chr11

119 Specific

FWD 5' TACAGCATCGCTCCGAAAGG 3'

REV 5' GGACAGAACCATGGCTGCTA 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001017677 · chr11

119 Specific

FWD 5' GGTTACAGCATCGCTCCGAA 3'

REV 5' ACCATGGCTGCTAGATGAGC 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001017677 · chr11

118 Specific

FWD 5' TACAGCATCGCTCCGAAAGG 3'

REV 5' GCTCCTGCTGAATGACCACA 3'

Fwd Tm 61.8°C

Rev Tm 62.2°C

GC 55%

Product 125 bp

Spans 2 introns

NM_001017677 · chr11

118 Specific

FWD 5' TACAGCATCGCTCCGAAAGG 3'

REV 5' GAGCTCCTGCTGAATGACCA 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 127 bp

Spans 2 introns

NM_001017677 · chr11

118 Specific

FWD 5' TACAGCATCGCTCCGAAAGG 3'

REV 5' TGAGCTCCTGCTGAATGACC 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 128 bp

Spans 2 introns

NM_001017677 · chr11

118 Specific

FWD 5' GCATCGCTCCGAAAGGTGAT 3'

REV 5' TGGCTGCTAGATGAGCTCCT 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 135 bp

Spans 2 introns