aftpha 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001145561 · chr1

118 Specific

FWD 5' TCTCCTTCAGTCGCCACAGA 3'

REV 5' TCGAGGTTGAGCAGAGACGA 3'

Fwd Tm 62.5°C

Rev Tm 62.4°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001145561 · chr1

117 Specific

FWD 5' GGACAGCACACAGGAGTCTC 3'

REV 5' GATTCATCCACTGGGCCGAA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 60%

Product 129 bp

Spans 2 introns

NM_001145561 · chr1

117 Specific

FWD 5' GAGTTGAGCTCCTGTCCACC 3'

REV 5' GATTCATCCACTGGGCCGAA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 60%

Product 149 bp

Spans 2 introns

NM_001145561 · chr1

117 Specific

FWD 5' CCTTCAGTCGCCACAGAGTT 3'

REV 5' TCGAGGTTGAGCAGAGACGA 3'

Fwd Tm 61.8°C

Rev Tm 62.4°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001145561 · chr1

117 Specific

FWD 5' CCACAGAGTTGAGCTCCTGT 3'

REV 5' ATCGAGGTTGAGCAGAGACG 3'

Fwd Tm 61.3°C

Rev Tm 61.2°C

GC 55%

Product 131 bp

Spans 2 introns

NM_001145561 · chr1

117 Specific

FWD 5' ATCTCCTTCAGTCGCCACAG 3'

REV 5' ATCGAGGTTGAGCAGAGACG 3'

Fwd Tm 61.3°C

Rev Tm 61.2°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001145561 · chr1

117 Specific

FWD 5' TCAGTCGCCACAGAGTTGAG 3'

REV 5' ATCGAGGTTGAGCAGAGACG 3'

Fwd Tm 61.4°C

Rev Tm 61.2°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001145561 · chr1

117 Specific

FWD 5' TCCTTCAGTCGCCACAGAGT 3'

REV 5' TCGAGGTTGAGCAGAGACGA 3'

Fwd Tm 62.8°C

Rev Tm 62.4°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001145561 · chr1

116 Specific

FWD 5' CCTTCAGTCGCCACAGAGTT 3'

REV 5' CGAGGTTGAGCAGAGACGAG 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001145561 · chr1

116 Specific

FWD 5' CCTTCAGTCGCCACAGAGTT 3'

REV 5' ATCGAGGTTGAGCAGAGACG 3'

Fwd Tm 61.8°C

Rev Tm 61.2°C

GC 55%

Product 141 bp

Spans 2 introns