agfg1a 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_199835 · chr15

120 Specific

FWD 5' CTCAACAACCCAACGGAGGT 3'

REV 5' CTCCTGCAGGAAACTGTGCT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 135 bp

Spans 2 introns

NM_199835 · chr15

119 Specific

FWD 5' CTCAACAACCCAACGGAGGT 3'

REV 5' TGGAACCTCCTGCAGGAAAC 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 141 bp

Spans 2 introns

NM_199835 · chr15

119 Specific

FWD 5' CTCAACAACCCAACGGAGGT 3'

REV 5' GTGGAACCTCCTGCAGGAAA 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 142 bp

Spans 2 introns

NM_199835 · chr15

119 Specific

FWD 5' CTCAACAACCCAACGGAGGT 3'

REV 5' GGTTTGTGGTGGAACCTCCT 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 150 bp

Spans 2 introns

NM_199835 · chr15

119 Specific

FWD 5' TCCTCAACAACCCAACGGAG 3'

REV 5' ACCTCCTGCAGGAAACTGTG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 139 bp

Spans 2 introns

NM_199835 · chr15

119 Specific

FWD 5' TCCTCAACAACCCAACGGAG 3'

REV 5' TGGAACCTCCTGCAGGAAAC 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 143 bp

Spans 2 introns

NM_199835 · chr15

119 Specific

FWD 5' TCCTCAACAACCCAACGGAG 3'

REV 5' GTGGAACCTCCTGCAGGAAA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 144 bp

Spans 2 introns

NM_199835 · chr15

119 Specific

FWD 5' GCATCCTACGTGGCCTGAAT 3'

REV 5' GCCAAGTTCCGGTCGTCATA 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 148 bp

Spans 2 introns

NM_199835 · chr15

119 Specific

FWD 5' CTCAACAACCCAACGGAGGT 3'

REV 5' ACCTCCTGCAGGAAACTGTG 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 137 bp

Spans 2 introns

NM_199835 · chr15

119 Specific

FWD 5' CAGCCGCATGCTTATCCTCA 3'

REV 5' TCCTGCAGGAAACTGTGCTG 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 150 bp

Spans 2 introns