agfg2 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001030238 · chr7_KZ115221v1_alt

118 Specific

FWD 5' CACGTCATGCTCCGGAATGT 3'

REV 5' AAATGTGCAGAGCCAGGTCC 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001030238 · chr7

118 Specific

FWD 5' CACGTCATGCTCCGGAATGT 3'

REV 5' AAATGTGCAGAGCCAGGTCC 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001030238 · chr7_KZ115221v1_alt

117 Specific

FWD 5' CACGTCATGCTCCGGAATGT 3'

REV 5' GGGTCAAATGTGCAGAGCCA 3'

Fwd Tm 62.4°C

Rev Tm 62.7°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001030238 · chr7

117 Specific

FWD 5' CACGTCATGCTCCGGAATGT 3'

REV 5' GGGTCAAATGTGCAGAGCCA 3'

Fwd Tm 62.4°C

Rev Tm 62.7°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001030238 · chr7_KZ115221v1_alt

116 Specific

FWD 5' GCTCCGGAATGTTGAGAGGA 3'

REV 5' CAAATGTGCAGAGCCAGGTC 3'

Fwd Tm 61.4°C

Rev Tm 61.2°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001030238 · chr7_KZ115221v1_alt

116 Specific

FWD 5' GCTCCGGAATGTTGAGAGGA 3'

REV 5' GTCAAATGTGCAGAGCCAGG 3'

Fwd Tm 61.4°C

Rev Tm 61.2°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001030238 · chr7_KZ115221v1_alt

116 Specific

FWD 5' GCTCCGGAATGTTGAGAGGA 3'

REV 5' GGTCAAATGTGCAGAGCCAG 3'

Fwd Tm 61.4°C

Rev Tm 61.2°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001030238 · chr7

116 Specific

FWD 5' GCTCCGGAATGTTGAGAGGA 3'

REV 5' CAAATGTGCAGAGCCAGGTC 3'

Fwd Tm 61.4°C

Rev Tm 61.2°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001030238 · chr7

116 Specific

FWD 5' GCTCCGGAATGTTGAGAGGA 3'

REV 5' GTCAAATGTGCAGAGCCAGG 3'

Fwd Tm 61.4°C

Rev Tm 61.2°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001030238 · chr7

116 Specific

FWD 5' GCTCCGGAATGTTGAGAGGA 3'

REV 5' GGTCAAATGTGCAGAGCCAG 3'

Fwd Tm 61.4°C

Rev Tm 61.2°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001030238 · chr7_KZ115221v1_alt

116 Specific

FWD 5' CGTCATGCTCCGGAATGTTG 3'

REV 5' CAAATGTGCAGAGCCAGGTC 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001030238 · chr7_KZ115221v1_alt

116 Specific

FWD 5' CGTCATGCTCCGGAATGTTG 3'

REV 5' GTCAAATGTGCAGAGCCAGG 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001030238 · chr7_KZ115221v1_alt

116 Specific

FWD 5' CGTCATGCTCCGGAATGTTG 3'

REV 5' GGTCAAATGTGCAGAGCCAG 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001030238 · chr7

116 Specific

FWD 5' CGTCATGCTCCGGAATGTTG 3'

REV 5' CAAATGTGCAGAGCCAGGTC 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001030238 · chr7

116 Specific

FWD 5' CGTCATGCTCCGGAATGTTG 3'

REV 5' GTCAAATGTGCAGAGCCAGG 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001030238 · chr7

116 Specific

FWD 5' CGTCATGCTCCGGAATGTTG 3'

REV 5' GGTCAAATGTGCAGAGCCAG 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001030238 · chr7_KZ115221v1_alt

116 Specific

FWD 5' TGCTCCGGAATGTTGAGAGG 3'

REV 5' CAAATGTGCAGAGCCAGGTC 3'

Fwd Tm 61.6°C

Rev Tm 61.2°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001030238 · chr7_KZ115221v1_alt

116 Specific

FWD 5' TGCTCCGGAATGTTGAGAGG 3'

REV 5' GTCAAATGTGCAGAGCCAGG 3'

Fwd Tm 61.6°C

Rev Tm 61.2°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001030238 · chr7

116 Specific

FWD 5' TGCTCCGGAATGTTGAGAGG 3'

REV 5' CAAATGTGCAGAGCCAGGTC 3'

Fwd Tm 61.6°C

Rev Tm 61.2°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001030238 · chr7

116 Specific

FWD 5' TGCTCCGGAATGTTGAGAGG 3'

REV 5' GTCAAATGTGCAGAGCCAGG 3'

Fwd Tm 61.6°C

Rev Tm 61.2°C

GC 55%

Product 141 bp

Spans 2 introns