agk 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_199880 · chr18

119 Specific

FWD 5' AAAGGGTGAGAAGGAGCAGC 3'

REV 5' CCAATGTGCTGCTCTCGTCT 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 127 bp

Spans 2 introns

NM_199880 · chr18

119 Specific

FWD 5' AAAGGGTGAGAAGGAGCAGC 3'

REV 5' TGCTGCTCTCGTCTTCAAGG 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 121 bp

Spans 2 introns

NM_199880 · chr18

119 Specific

FWD 5' GTTCCACTGGACGTACTGCA 3'

REV 5' TGCTGCTCTCGTCTTCAAGG 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 144 bp

Spans 2 introns

NM_199880 · chr18

119 Specific

FWD 5' AACGGTTCCACTGGACGTAC 3'

REV 5' TGCTGCTCTCGTCTTCAAGG 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 148 bp

Spans 2 introns

NM_199880 · chr18

118 Specific

FWD 5' ACATCTGGCTGGTGTGGAAG 3'

REV 5' CTACGAAGCAGGCCGGTTAT 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 150 bp

Spans 2 introns

NM_199880 · chr18

118 Specific

FWD 5' CATCTGGCTGGTGTGGAAGT 3'

REV 5' CTACGAAGCAGGCCGGTTAT 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 149 bp

Spans 2 introns

NM_199880 · chr18

118 Specific

FWD 5' ACATCTGGCTGGTGTGGAAG 3'

REV 5' TACGAAGCAGGCCGGTTATC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 149 bp

Spans 2 introns

NM_199880 · chr18

118 Specific

FWD 5' CATCTGGCTGGTGTGGAAGT 3'

REV 5' TACGAAGCAGGCCGGTTATC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 148 bp

Spans 2 introns

NM_199880 · chr18

118 Specific

FWD 5' TCCACTGGACGTACTGCAGA 3'

REV 5' ACCAATGTGCTGCTCTCGTC 3'

Fwd Tm 62.5°C

Rev Tm 62.3°C

GC 55%

Product 149 bp

Spans 2 introns

NM_199880 · chr18

118 Specific

FWD 5' GCGCTGTTGGGATTACGTTG 3'

REV 5' AGGGAAGCCACATGAACCTG 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 143 bp

Spans 2 introns