ago1 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001317223 · chr16

118 Specific

FWD 5' TCAAAGGCCTGAAGGTGGAG 3'

REV 5' TGCATTCTACCGTCTGTCCG 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 135 bp

Spans 2 introns

NM_001317223 · chr16

118 Specific

FWD 5' AGGCCTGAAGGTGGAGGTTA 3'

REV 5' TTCTACCGTCTGTCCGCTCT 3'

Fwd Tm 62.5°C

Rev Tm 62.2°C

GC 55%

Product 127 bp

Spans 2 introns

NM_001317223 · chr16

118 Specific

FWD 5' GCGGCATCAACAACATCCTC 3'

REV 5' GATGAGCGTCCATACTGCCA 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001317223 · chr16

117 Specific

FWD 5' TCAACAACATCCTCGTGCCG 3'

REV 5' GAGCGTCCATACTGCCAACA 3'

Fwd Tm 62.5°C

Rev Tm 62.1°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001317223 · chr16

117 Specific

FWD 5' TCAACAACATCCTCGTGCCG 3'

REV 5' TGAGCGTCCATACTGCCAAC 3'

Fwd Tm 62.5°C

Rev Tm 62.1°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001317223 · chr16

117 Specific

FWD 5' GCGGCATCAACAACATCCTC 3'

REV 5' GCGTCCATACTGCCAACAAC 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001317223 · chr16

117 Specific

FWD 5' TCAAAGGCCTGAAGGTGGAG 3'

REV 5' TTCTACCGTCTGTCCGCTCT 3'

Fwd Tm 61.6°C

Rev Tm 62.2°C

GC 55%

Product 131 bp

Spans 2 introns

NM_001317223 · chr16

117 Specific

FWD 5' CCGGTTCACCAAGGAGATCA 3'

REV 5' CATTCTACCGTCTGTCCGCT 3'

Fwd Tm 61.3°C

Rev Tm 61.3°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001317223 · chr16

117 Specific

FWD 5' CGGTCAGCAGTGTTTCAGCA 3'

REV 5' TAGCGCAGTATCTGCTGGGA 3'

Fwd Tm 62.8°C

Rev Tm 62.7°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001317223 · chr16

117 Specific

FWD 5' ACGGTCAGCAGTGTTTCAGC 3'

REV 5' TAGCGCAGTATCTGCTGGGA 3'

Fwd Tm 62.8°C

Rev Tm 62.7°C

GC 55%

Product 137 bp

Spans 2 introns