agtrap 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001201345 · chr11_KZ115346v1_alt

116 Specific

FWD 5' ACTGACAGTTTGGGCATGCT 3'

REV 5' GTCACTGCCAGTCCGATCAA 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 50%

Product 147 bp

Spans 2 introns

NM_001201345 · chr11_KZ115346v1_alt

116 Specific

FWD 5' ACTGACAGTTTGGGCATGCT 3'

REV 5' TTGTCACTGCCAGTCCGATC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 50%

Product 149 bp

Spans 2 introns

NM_001201345 · chr11

116 Specific

FWD 5' ACTGACAGTTTGGGCATGCT 3'

REV 5' GTCACTGCCAGTCCGATCAA 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 50%

Product 147 bp

Spans 2 introns

NM_001201345 · chr11

116 Specific

FWD 5' ACTGACAGTTTGGGCATGCT 3'

REV 5' TTGTCACTGCCAGTCCGATC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 50%

Product 149 bp

Spans 2 introns

NM_001201345 · chr11_KZ115346v1_alt

115 Specific

FWD 5' ACTGACAGTTTGGGCATGCT 3'

REV 5' CACTGCCAGTCCGATCAAGA 3'

Fwd Tm 62.0°C

Rev Tm 61.5°C

GC 50%

Product 145 bp

Spans 2 introns

NM_001201345 · chr11_KZ115346v1_alt

115 Specific

FWD 5' ACTGACAGTTTGGGCATGCT 3'

REV 5' TCACTGCCAGTCCGATCAAG 3'

Fwd Tm 62.0°C

Rev Tm 61.5°C

GC 50%

Product 146 bp

Spans 2 introns

NM_001201345 · chr11

115 Specific

FWD 5' ACTGACAGTTTGGGCATGCT 3'

REV 5' CACTGCCAGTCCGATCAAGA 3'

Fwd Tm 62.0°C

Rev Tm 61.5°C

GC 50%

Product 145 bp

Spans 2 introns

NM_001201345 · chr11

115 Specific

FWD 5' ACTGACAGTTTGGGCATGCT 3'

REV 5' TCACTGCCAGTCCGATCAAG 3'

Fwd Tm 62.0°C

Rev Tm 61.5°C

GC 50%

Product 146 bp

Spans 2 introns

NM_001201345 · chr11_KZ115346v1_alt

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' CACTGCCAGTCCGATCAAGA 3'

Fwd Tm 60.8°C

Rev Tm 61.5°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001201345 · chr11_KZ115346v1_alt

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' TCACTGCCAGTCCGATCAAG 3'

Fwd Tm 60.8°C

Rev Tm 61.5°C

GC 55%

Product 135 bp

Spans 2 introns

NM_001201345 · chr11

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' CACTGCCAGTCCGATCAAGA 3'

Fwd Tm 60.8°C

Rev Tm 61.5°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001201345 · chr11

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' TCACTGCCAGTCCGATCAAG 3'

Fwd Tm 60.8°C

Rev Tm 61.5°C

GC 55%

Product 135 bp

Spans 2 introns

NM_001201345 · chr11_KZ115346v1_alt

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' GTCACTGCCAGTCCGATCAA 3'

Fwd Tm 60.8°C

Rev Tm 61.8°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001201345 · chr11_KZ115346v1_alt

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' TTGTCACTGCCAGTCCGATC 3'

Fwd Tm 60.8°C

Rev Tm 61.8°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001201345 · chr11_KZ115346v1_alt

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' GATTGTCACTGCCAGTCCGA 3'

Fwd Tm 60.8°C

Rev Tm 61.8°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001201345 · chr11

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' GTCACTGCCAGTCCGATCAA 3'

Fwd Tm 60.8°C

Rev Tm 61.8°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001201345 · chr11

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' TTGTCACTGCCAGTCCGATC 3'

Fwd Tm 60.8°C

Rev Tm 61.8°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001201345 · chr11

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' GATTGTCACTGCCAGTCCGA 3'

Fwd Tm 60.8°C

Rev Tm 61.8°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001201345 · chr11_KZ115346v1_alt

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' AGATTGTCACTGCCAGTCCG 3'

Fwd Tm 60.8°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001201345 · chr11

114 Specific

FWD 5' GGGCATGCTTACTTTCCTGG 3'

REV 5' AGATTGTCACTGCCAGTCCG 3'

Fwd Tm 60.8°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 2 introns