ahcyl2a 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001080649 · chr25

119 Specific

FWD 5' TGATTCTTCTGGCAGAGGGC 3'

REV 5' GCCCTCAGGAGCATTGAAGA 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 125 bp

Spans 2 introns

NM_001080649 · chr25

119 Specific

FWD 5' CTGCTGCAGGGAGTCCATTT 3'

REV 5' TTCAGAGCAGCAGAACAGCC 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 120 bp

Spans 2 introns

NM_001080649 · chr25

118 Specific

FWD 5' CTGCTGCAGGGAGTCCATTT 3'

REV 5' CAGAGCAGCAGAACAGCCTT 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 118 bp

Spans 2 introns

NM_001080649 · chr25

118 Specific

FWD 5' TGATTCTTCTGGCAGAGGGC 3'

REV 5' TCTTGCTTGTAACGGCCCTC 3'

Fwd Tm 61.7°C

Rev Tm 62.1°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001080649 · chr25

118 Specific

FWD 5' TTGTACTGCTGCAGGGAGTC 3'

REV 5' CCCATTGCTTTCAGAGCAGC 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001080649 · chr25

118 Specific

FWD 5' ACTTGTACTGCTGCAGGGAG 3'

REV 5' CCCATTGCTTTCAGAGCAGC 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001080649 · chr25

118 Specific

FWD 5' CTTGTACTGCTGCAGGGAGT 3'

REV 5' CCCATTGCTTTCAGAGCAGC 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 135 bp

Spans 2 introns

NM_001080649 · chr25

117 Specific

FWD 5' CAGAGGGCCGTGTCCTTAAT 3'

REV 5' GCCCTCAGGAGCATTGAAGA 3'

Fwd Tm 61.4°C

Rev Tm 61.7°C

GC 55%

Product 113 bp

Spans 2 introns

NM_001080649 · chr25

117 Specific

FWD 5' CTGCAAGCATGCATGGATGG 3'

REV 5' ATCCATGTGTTCCCGCACAA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 117 bp

Spans 2 introns

NM_001080649 · chr25

117 Specific

FWD 5' GTGCTCTGCAAGCATGCATG 3'

REV 5' CATCCATGTGTTCCCGCACA 3'

Fwd Tm 62.3°C

Rev Tm 62.7°C

GC 55%

Product 123 bp

Spans 2 introns