ahi1 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001319989 · chr17

118 Specific

FWD 5' TCCATCCCTCAGACAGCAGA 3'

REV 5' TCGTCCGACCTATTGGCTCT 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 126 bp

Spans 2 introns

NM_001319989 · chr17

118 Specific

FWD 5' CACACTGACCTCACACACCA 3'

REV 5' ACCGTCTGATGAGCTTGACC 3'

Fwd Tm 61.7°C

Rev Tm 61.5°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001319989 · chr17

118 Specific

FWD 5' CACACTGACCTCACACACCA 3'

REV 5' CGTCTGATGAGCTTGACCCA 3'

Fwd Tm 61.7°C

Rev Tm 61.5°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001319989 · chr17

118 Specific

FWD 5' GACCTCACACACCAGTGGTT 3'

REV 5' ACCGTCTGATGAGCTTGACC 3'

Fwd Tm 61.7°C

Rev Tm 61.5°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001319989 · chr17

118 Specific

FWD 5' GACCTCACACACCAGTGGTT 3'

REV 5' CGTCTGATGAGCTTGACCCA 3'

Fwd Tm 61.7°C

Rev Tm 61.5°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001319989 · chr17

118 Specific

FWD 5' AGTGGTTACAGTCAAGCCGG 3'

REV 5' ACCGTCTGATGAGCTTGACC 3'

Fwd Tm 61.8°C

Rev Tm 61.5°C

GC 55%

Product 125 bp

Spans 2 introns

NM_001319989 · chr17

118 Specific

FWD 5' AGTGGTTACAGTCAAGCCGG 3'

REV 5' CGTCTGATGAGCTTGACCCA 3'

Fwd Tm 61.8°C

Rev Tm 61.5°C

GC 55%

Product 123 bp

Spans 2 introns

NM_001319989 · chr17

118 Specific

FWD 5' AGAGCTCCATCCCTCAGACA 3'

REV 5' TCGTCCGACCTATTGGCTCT 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 131 bp

Spans 2 introns

NM_001319989 · chr17

118 Specific

FWD 5' ACACTGACCTCACACACCAG 3'

REV 5' CGTCTGATGAGCTTGACCCA 3'

Fwd Tm 61.4°C

Rev Tm 61.5°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001319989 · chr17

118 Specific

FWD 5' CACTGACCTCACACACCAGT 3'

REV 5' CGTCTGATGAGCTTGACCCA 3'

Fwd Tm 61.4°C

Rev Tm 61.5°C

GC 55%

Product 140 bp

Spans 2 introns