aida 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_200790 · chr20

119 Specific

FWD 5' AAATACTCGCCAGGCAGCTC 3'

REV 5' GGCATGTGGCAAACTTTCCC 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 99 bp

Spans 2 introns

NM_200790 · chr20

118 Specific

FWD 5' CTGGTGGAAGCCATCGATGA 3'

REV 5' GGCATGTGGCAAACTTTCCC 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 124 bp

Spans 2 introns

NM_200790 · chr20

118 Specific

FWD 5' TCAAATACTCGCCAGGCAGC 3'

REV 5' GGCATGTGGCAAACTTTCCC 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 101 bp

Spans 2 introns

NM_200790 · chr20

118 Specific

FWD 5' CAAATACTCGCCAGGCAGCT 3'

REV 5' CGCATTTCAAGGCATGTGGC 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 110 bp

Spans 2 introns

NM_200790 · chr20

118 Specific

FWD 5' TCAAATACTCGCCAGGCAGC 3'

REV 5' CGCATTTCAAGGCATGTGGC 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 111 bp

Spans 2 introns

NM_200790 · chr20

118 Specific

FWD 5' CAAATACTCGCCAGGCAGCT 3'

REV 5' CTGTAAGGCTGCACTTCGCA 3'

Fwd Tm 62.5°C

Rev Tm 62.6°C

GC 55%

Product 126 bp

Spans 2 introns

NM_200790 · chr20

118 Specific

FWD 5' CAAATACTCGCCAGGCAGCT 3'

REV 5' GGCATGTGGCAAACTTTCCC 3'

Fwd Tm 62.5°C

Rev Tm 62.0°C

GC 55%

Product 100 bp

Spans 2 introns

NM_200790 · chr20

118 Specific

FWD 5' TCCAACCAGTGCCTTTGAGG 3'

REV 5' CGAGGGCAAACGTGGTAACA 3'

Fwd Tm 62.1°C

Rev Tm 62.5°C

GC 55%

Product 149 bp

Spans 2 introns

NM_200790 · chr20

118 Specific

FWD 5' CCAACCAGTGCCTTTGAGGA 3'

REV 5' CGAGGGCAAACGTGGTAACA 3'

Fwd Tm 62.1°C

Rev Tm 62.5°C

GC 55%

Product 148 bp

Spans 2 introns

NM_200790 · chr20

118 Specific

FWD 5' TCAAATACTCGCCAGGCAGC 3'

REV 5' CTGTAAGGCTGCACTTCGCA 3'

Fwd Tm 62.4°C

Rev Tm 62.6°C

GC 55%

Product 127 bp

Spans 2 introns