aifm1 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_200102 · chr5

118 Specific

FWD 5' TGTGCTCACCGACAGTAAGG 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 61.4°C

Rev Tm 61.4°C

GC 55%

Product 132 bp

Spans 2 introns

NM_200102 · chr5

118 Specific

FWD 5' TGCTCACCGACAGTAAGGTG 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 61.4°C

Rev Tm 61.4°C

GC 55%

Product 130 bp

Spans 2 introns

NM_200102 · chr5_KZ115110v1_alt

118 Specific

FWD 5' TGTGCTCACCGACAGTAAGG 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 61.4°C

Rev Tm 61.4°C

GC 55%

Product 132 bp

Spans 2 introns

NM_200102 · chr5_KZ115110v1_alt

118 Specific

FWD 5' TGCTCACCGACAGTAAGGTG 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 61.4°C

Rev Tm 61.4°C

GC 55%

Product 130 bp

Spans 2 introns

NM_200102 · chr5

118 Specific

FWD 5' GTGCTCACCGACAGTAAGGT 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 55%

Product 131 bp

Spans 2 introns

NM_200102 · chr5_KZ115110v1_alt

118 Specific

FWD 5' GTGCTCACCGACAGTAAGGT 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 55%

Product 131 bp

Spans 2 introns

NM_200102 · chr5

117 Specific

FWD 5' CGACAGTAAGGTGGTGCACA 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 62.0°C

Rev Tm 61.4°C

GC 55%

Product 123 bp

Spans 2 introns

NM_200102 · chr5_KZ115110v1_alt

117 Specific

FWD 5' CGACAGTAAGGTGGTGCACA 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 62.0°C

Rev Tm 61.4°C

GC 55%

Product 123 bp

Spans 2 introns

NM_200102 · chr5

117 Specific

FWD 5' GCTGTGCTCACCGACAGTAA 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 62.0°C

Rev Tm 61.4°C

GC 55%

Product 134 bp

Spans 2 introns

NM_200102 · chr5_KZ115110v1_alt

117 Specific

FWD 5' GCTGTGCTCACCGACAGTAA 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 62.0°C

Rev Tm 61.4°C

GC 55%

Product 134 bp

Spans 2 introns

NM_200102 · chr5

117 Specific

FWD 5' GGACCACTGAGAAAGTCCGG 3'

REV 5' GCTGCCACTATGTGGTCTGT 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 60%

Product 139 bp

Spans 2 introns

NM_200102 · chr5

117 Specific

FWD 5' GGACCACTGAGAAAGTCCGG 3'

REV 5' ACTGCTGCCACTATGTGGTC 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 60%

Product 142 bp

Spans 2 introns

NM_200102 · chr5_KZ115110v1_alt

117 Specific

FWD 5' GGACCACTGAGAAAGTCCGG 3'

REV 5' GCTGCCACTATGTGGTCTGT 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 60%

Product 139 bp

Spans 2 introns

NM_200102 · chr5_KZ115110v1_alt

117 Specific

FWD 5' GGACCACTGAGAAAGTCCGG 3'

REV 5' ACTGCTGCCACTATGTGGTC 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 60%

Product 142 bp

Spans 2 introns

NM_200102 · chr5

117 Specific

FWD 5' GGACCACTGAGAAAGTCCGG 3'

REV 5' TGCTGCCACTATGTGGTCTG 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 60%

Product 140 bp

Spans 2 introns

NM_200102 · chr5_KZ115110v1_alt

117 Specific

FWD 5' GGACCACTGAGAAAGTCCGG 3'

REV 5' TGCTGCCACTATGTGGTCTG 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 60%

Product 140 bp

Spans 2 introns

NM_200102 · chr5

116 Specific

FWD 5' TCACCGACAGTAAGGTGGTG 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 61.2°C

Rev Tm 61.4°C

GC 55%

Product 127 bp

Spans 2 introns

NM_200102 · chr5_KZ115110v1_alt

116 Specific

FWD 5' TCACCGACAGTAAGGTGGTG 3'

REV 5' ATAACCTGCAGGTTCCTCGG 3'

Fwd Tm 61.2°C

Rev Tm 61.4°C

GC 55%

Product 127 bp

Spans 2 introns

NM_200102 · chr5

116 Specific

FWD 5' TGGACCACTGAGAAAGTCCG 3'

REV 5' CTGCTGCCACTATGTGGTCT 3'

Fwd Tm 61.2°C

Rev Tm 61.6°C

GC 55%

Product 142 bp

Spans 2 introns

NM_200102 · chr5_KZ115110v1_alt

116 Specific

FWD 5' TGGACCACTGAGAAAGTCCG 3'

REV 5' CTGCTGCCACTATGTGGTCT 3'

Fwd Tm 61.2°C

Rev Tm 61.6°C

GC 55%

Product 142 bp

Spans 2 introns