aire 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001110014 · chr2

119 Specific

FWD 5' ACAGGACACATCACCACCAC 3'

REV 5' TGGACTGAGGTGGAAACTGC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001110014 · chr2

119 Specific

FWD 5' CACAGGACACATCACCACCA 3'

REV 5' TGGACTGAGGTGGAAACTGC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001110014 · chr2

119 Specific

FWD 5' CAGGACACATCACCACCACA 3'

REV 5' TGGACTGAGGTGGAAACTGC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001110014 · chr2

119 Specific

FWD 5' TCCAGCTCTGGAAGCAAAGG 3'

REV 5' TGGACTGAGGTGGAAACTGC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 104 bp

Spans 2 introns

NM_001110014 · chr2

119 Specific

FWD 5' TCCAGCTCTGGAAGCAAAGG 3'

REV 5' AGATACCGTCACAGCCCTCT 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 123 bp

Spans 2 introns

NM_001110014 · chr2

119 Specific

FWD 5' CTGGAAGCAAAGGCAAGCTG 3'

REV 5' TGGACTGAGGTGGAAACTGC 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 97 bp

Spans 2 introns

NM_001110014 · chr2

118 Specific

FWD 5' AGGACACATCACCACCACAG 3'

REV 5' GGACTGAGGTGGAAACTGCT 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001110014 · chr2

118 Specific

FWD 5' ATCACGCCCTCTGCAACTTC 3'

REV 5' TGCTCTGATGGCGTTAAGCC 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001110014 · chr2

118 Specific

FWD 5' ATCACGCCCTCTGCAACTTC 3'

REV 5' TGGCGTTAAGCCCTGATCAC 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001110014 · chr2

118 Specific

FWD 5' CTGGAAGCAAAGGCAAGCTG 3'

REV 5' GGACTGAGGTGGAAACTGCT 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 96 bp

Spans 2 introns