ak2 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_212596 · chr19

119 Specific

FWD 5' AGGCTCATTCATCAGCCCAG 3'

REV 5' TCTAGTCGAGAGCGCAGAGT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 140 bp

Spans 2 introns

NM_212596 · chr19

119 Specific

FWD 5' AGGCTCATTCATCAGCCCAG 3'

REV 5' ACTCTAGTCGAGAGCGCAGA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 142 bp

Spans 2 introns

NM_212596 · chr19

119 Specific

FWD 5' AGGCTCATTCATCAGCCCAG 3'

REV 5' AGACTCTAGTCGAGAGCGCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 144 bp

Spans 2 introns

NM_212596 · chr19

119 Specific

FWD 5' TCTGTCGATGACTCCCTGCT 3'

REV 5' TACGGCGGATCAGAGGTTCT 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 142 bp

Spans 2 introns

NM_212596 · chr19

118 Specific

FWD 5' TAGTCCGCAGGATCTGTGGA 3'

REV 5' TACGGCGGATCAGAGGTTCT 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 123 bp

Spans 2 introns

NM_212596 · chr19

118 Specific

FWD 5' AGGCTCATTCATCAGCCCAG 3'

REV 5' AGTCGAGAGCGCAGAGTAGT 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 137 bp

Spans 2 introns

NM_212596 · chr19

117 Specific

FWD 5' TGCTAGTCCGCAGGATCTGT 3'

REV 5' ACTACGGCGGATCAGAGGTT 3'

Fwd Tm 62.6°C

Rev Tm 62.6°C

GC 55%

Product 128 bp

Spans 2 introns

NM_212596 · chr19

117 Specific

FWD 5' AGGCTCATTCATCAGCCCAG 3'

REV 5' TACGGCGGATCAGAGGTTCT 3'

Fwd Tm 61.8°C

Rev Tm 62.3°C

GC 55%

Product 103 bp

Spans 2 introns

NM_212596 · chr19

117 Specific

FWD 5' TCTGTCGATGACTCCCTGCT 3'

REV 5' ACTACGGCGGATCAGAGGTT 3'

Fwd Tm 62.3°C

Rev Tm 62.6°C

GC 55%

Product 144 bp

Spans 2 introns

NM_212596 · chr19

117 Specific

FWD 5' TGCTAGTCCGCAGGATCTGT 3'

REV 5' TACGGCGGATCAGAGGTTCT 3'

Fwd Tm 62.6°C

Rev Tm 62.3°C

GC 55%

Product 126 bp

Spans 2 introns