ak3 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_213130 · chr10

109 Specific

FWD 5' GAGACATGCTGAGGGCCAAT 3'

REV 5' ACACAATCCAGAGCCTCAGC 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 199 bp

Spans 2 introns

NM_213130 · chr10

109 Specific

FWD 5' GGCCAATATCGAGGCCAAGA 3'

REV 5' ACACAATCCAGAGCCTCAGC 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 186 bp

Spans 2 introns

NM_213130 · chr10

108 Specific

FWD 5' ATCTCGCTGGGTGCATCTTC 3'

REV 5' CAGCGGCTCTCCTGTTACAT 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 55%

Product 97 bp

Spans 1 intron

NM_213130 · chr10

108 Specific

FWD 5' ATCTCGCTGGGTGCATCTTC 3'

REV 5' GCGGCTCTCCTGTTACATCA 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 55%

Product 95 bp

Spans 1 intron

NM_213130 · chr10

108 Specific

FWD 5' TACTGGACGGGTTTCCTCGT 3'

REV 5' GAAAGGCACGTCCAGGTTGA 3'

Fwd Tm 62.5°C

Rev Tm 62.3°C

GC 55%

Product 92 bp

Spans 1 intron

NM_213130 · chr10

108 Specific

FWD 5' ACTGGACGGGTTTCCTCGTA 3'

REV 5' GAAAGGCACGTCCAGGTTGA 3'

Fwd Tm 62.5°C

Rev Tm 62.3°C

GC 55%

Product 91 bp

Spans 1 intron

NM_213130 · chr10

108 Specific

FWD 5' TACTGGACGGGTTTCCTCGT 3'

REV 5' AAGATGCACCCAGCGAGATG 3'

Fwd Tm 62.5°C

Rev Tm 62.2°C

GC 55%

Product 134 bp

Spans 1 intron

NM_213130 · chr10

108 Specific

FWD 5' ACTGGACGGGTTTCCTCGTA 3'

REV 5' AAGATGCACCCAGCGAGATG 3'

Fwd Tm 62.5°C

Rev Tm 62.2°C

GC 55%

Product 133 bp

Spans 1 intron

NM_213130 · chr10

108 Specific

FWD 5' TGGACGGGTTTCCTCGTACT 3'

REV 5' AAGATGCACCCAGCGAGATG 3'

Fwd Tm 62.5°C

Rev Tm 62.2°C

GC 55%

Product 131 bp

Spans 1 intron

NM_213130 · chr10

108 Specific

FWD 5' TGCTGAGGGCCAATATCGAG 3'

REV 5' ACACAATCCAGAGCCTCAGC 3'

Fwd Tm 61.5°C

Rev Tm 61.9°C

GC 55%

Product 193 bp

Spans 2 introns