ak4 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_213299 · chr6

109 Specific

FWD 5' CGACTGCGACATCGATGGAT 3'

REV 5' CTCCTGCTGAATCAGTGCCT 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 117 bp

Spans 1 intron

NM_213299 · chr6

108 Specific

FWD 5' GAGGATCGCGCACAATTTCG 3'

REV 5' ATGGTCTGGAACCAGCAGTC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 133 bp

Spans 1 intron

NM_213299 · chr6

108 Specific

FWD 5' TGCTGGTTCCAGACCATGTG 3'

REV 5' TTCAGAGCTTCAGCCTGTGC 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 118 bp

Spans 1 intron

NM_213299 · chr6

108 Specific

FWD 5' CGACTGCGACATCGATGGAT 3'

REV 5' TCCTGCTGAATCAGTGCCTC 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 116 bp

Spans 1 intron

NM_213299 · chr6

108 Specific

FWD 5' CGACTGCGACATCGATGGAT 3'

REV 5' TCCTCCTGCTGAATCAGTGC 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 119 bp

Spans 1 intron

NM_213299 · chr6

108 Specific

FWD 5' GAGGATCGCGCACAATTTCG 3'

REV 5' GTCTGGAACCAGCAGTCCTT 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 130 bp

Spans 1 intron

NM_213299 · chr6

108 Specific

FWD 5' ACCCAGTGGGAGAGTGTACA 3'

REV 5' CTCCTGCTGAATCAGTGCCT 3'

Fwd Tm 62.0°C

Rev Tm 61.7°C

GC 55%

Product 91 bp

Spans 1 intron

NM_213299 · chr6

108 Specific

FWD 5' GAGGATCGCGCACAATTTCG 3'

REV 5' TCACATGGTCTGGAACCAGC 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 137 bp

Spans 1 intron

NM_213299 · chr6

108 Specific

FWD 5' AACGACTGCGACATCGATGG 3'

REV 5' TGGTCTGTCATCCTCCTGCT 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 131 bp

Spans 1 intron

NM_213299 · chr6

108 Specific

FWD 5' GAGGATCGCGCACAATTTCG 3'

REV 5' CATGGTCTGGAACCAGCAGT 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 134 bp

Spans 1 intron