akap12b 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001281788 · chr20

116 Specific

FWD 5' GAGAAACCGCAGATGGCAAG 3'

REV 5' GGGAGATGGTGTCCGTTTGA 3'

Fwd Tm 61.1°C

Rev Tm 61.6°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001281788 · chr20

115 Specific

FWD 5' GCAGATGGCAAGCCTCTTCA 3'

REV 5' GGGAGATGGTGTCCGTTTGA 3'

Fwd Tm 62.5°C

Rev Tm 61.6°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001281788 · chr20

115 Specific

FWD 5' ATGGAGAAACCGCAGATGGC 3'

REV 5' GGGAGATGGTGTCCGTTTGA 3'

Fwd Tm 62.5°C

Rev Tm 61.6°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001281788 · chr20

114 Specific

FWD 5' GGAGAAACCGCAGATGGCAA 3'

REV 5' GGGAGATGGTGTCCGTTTGA 3'

Fwd Tm 62.7°C

Rev Tm 61.6°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001281788 · chr20

114 Specific

FWD 5' GAGAAACCGCAGATGGCAAG 3'

REV 5' AGATGGTGTCCGTTTGACCA 3'

Fwd Tm 61.1°C

Rev Tm 61.0°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001281788 · chr20

113 Specific

FWD 5' CAGATGGCAAGCCTCTTCAG 3'

REV 5' GAGATGGTGTCCGTTTGACC 3'

Fwd Tm 60.4°C

Rev Tm 60.3°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001281788 · chr20

113 Specific

FWD 5' CAGATGGCAAGCCTCTTCAG 3'

REV 5' GGAGATGGTGTCCGTTTGAC 3'

Fwd Tm 60.4°C

Rev Tm 60.3°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001281788 · chr20

113 Specific

FWD 5' GATGGAGAAACCGCAGATGG 3'

REV 5' GAGATGGTGTCCGTTTGACC 3'

Fwd Tm 60.4°C

Rev Tm 60.3°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001281788 · chr20

113 Specific

FWD 5' GATGGAGAAACCGCAGATGG 3'

REV 5' GGAGATGGTGTCCGTTTGAC 3'

Fwd Tm 60.4°C

Rev Tm 60.3°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001281788 · chr20

112 Specific

FWD 5' CAGATGGCAAGCCTCTTCAG 3'

REV 5' GGGAGATGGTGTCCGTTTGA 3'

Fwd Tm 60.4°C

Rev Tm 61.6°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001098184 · chr20

109 1 gene · 2 transcripts

FWD 5' TGAGATCGAGCCTGTCTCCA 3'

REV 5' TAATGGCCGCCACTCACTTC 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 120 bp

Spans 1 intron

NM_001098184 · chr20

109 1 gene · 2 transcripts

FWD 5' TGAGATCGAGCCTGTCTCCA 3'

REV 5' CTTAATGGCCGCCACTCACT 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 122 bp

Spans 1 intron

NM_001098184 · chr20

109 1 gene · 2 transcripts

FWD 5' ACAGTGTCATGAGCAGTCGG 3'

REV 5' TAGTGTCCTTAATGGCCGCC 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001098184 · chr20

109 1 gene · 2 transcripts

FWD 5' CATGAGCAGTCGGCCTGTAA 3'

REV 5' TAGTGTCCTTAATGGCCGCC 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 91 bp

Spans 1 intron

NM_001098184 · chr20

109 1 gene · 2 transcripts

FWD 5' AGATCGAGCCTGTCTCCACA 3'

REV 5' CTTAATGGCCGCCACTCACT 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 120 bp

Spans 1 intron

NM_001098184 · chr20

109 1 gene · 2 transcripts

FWD 5' ATCGAGCCTGTCTCCACAGA 3'

REV 5' CTTAATGGCCGCCACTCACT 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001098184 · chr20

109 1 gene · 2 transcripts

FWD 5' AGATCGAGCCTGTCTCCACA 3'

REV 5' TAATGGCCGCCACTCACTTC 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001098184 · chr20

109 1 gene · 2 transcripts

FWD 5' ATCGAGCCTGTCTCCACAGA 3'

REV 5' TAATGGCCGCCACTCACTTC 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 116 bp

Spans 1 intron

NM_001098184 · chr20

108 1 gene · 2 transcripts

FWD 5' TGAGATCGAGCCTGTCTCCA 3'

REV 5' TAGTGTCCTTAATGGCCGCC 3'

Fwd Tm 62.0°C

Rev Tm 61.7°C

GC 55%

Product 129 bp

Spans 1 intron

NM_001098184 · chr20

108 1 gene · 2 transcripts

FWD 5' AACTGGAGGCTGCTTCAGAG 3'

REV 5' GCTCTGTGTCCCAGTCACAT 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 144 bp

Spans 1 intron