akip1 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001044983 · chr18

118 Specific

FWD 5' TCAGAGGGAGATACCTGCCA 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 113 bp

Spans 2 introns

NM_001044983 · chr18_KZ115554v1_alt

118 Specific

FWD 5' TCAGAGGGAGATACCTGCCA 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 113 bp

Spans 2 introns

NM_001044983 · chr18

117 Specific

FWD 5' TCAGAGGGAGATACCTGCCA 3'

REV 5' ATGTGCTCTTGCTCACTGGG 3'

Fwd Tm 61.6°C

Rev Tm 62.2°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001044983 · chr18_KZ115554v1_alt

117 Specific

FWD 5' TCAGAGGGAGATACCTGCCA 3'

REV 5' ATGTGCTCTTGCTCACTGGG 3'

Fwd Tm 61.6°C

Rev Tm 62.2°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001044983 · chr18

116 Specific

FWD 5' GGGCTCAGAGGGAGATACCT 3'

REV 5' ATGTGCTCTTGCTCACTGGG 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 60%

Product 141 bp

Spans 2 introns

NM_001044983 · chr18_KZ115554v1_alt

116 Specific

FWD 5' GGGCTCAGAGGGAGATACCT 3'

REV 5' ATGTGCTCTTGCTCACTGGG 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 60%

Product 141 bp

Spans 2 introns

NM_001044983 · chr18

116 Specific

FWD 5' GACCAACTCTCGGGTTCTGT 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.2°C

Rev Tm 61.8°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001044983 · chr18

116 Specific

FWD 5' ACCAACTCTCGGGTTCTGTC 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.2°C

Rev Tm 61.8°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001044983 · chr18

116 Specific

FWD 5' CCAACTCTCGGGTTCTGTCA 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.2°C

Rev Tm 61.8°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001044983 · chr18

116 Specific

FWD 5' TCTCGGGTTCTGTCACAAGG 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.2°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001044983 · chr18

116 Specific

FWD 5' CTCGGGTTCTGTCACAAGGA 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.2°C

Rev Tm 61.8°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001044983 · chr18_KZ115554v1_alt

116 Specific

FWD 5' GACCAACTCTCGGGTTCTGT 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.2°C

Rev Tm 61.8°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001044983 · chr18_KZ115554v1_alt

116 Specific

FWD 5' ACCAACTCTCGGGTTCTGTC 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.2°C

Rev Tm 61.8°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001044983 · chr18_KZ115554v1_alt

116 Specific

FWD 5' CCAACTCTCGGGTTCTGTCA 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.2°C

Rev Tm 61.8°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001044983 · chr18_KZ115554v1_alt

116 Specific

FWD 5' TCTCGGGTTCTGTCACAAGG 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.2°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001044983 · chr18_KZ115554v1_alt

116 Specific

FWD 5' CTCGGGTTCTGTCACAAGGA 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.2°C

Rev Tm 61.8°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001044983 · chr18

115 Specific

FWD 5' GGGCTCAGAGGGAGATACCT 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 62.3°C

Rev Tm 61.8°C

GC 60%

Product 117 bp

Spans 2 introns

NM_001044983 · chr18_KZ115554v1_alt

115 Specific

FWD 5' GGGCTCAGAGGGAGATACCT 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 62.3°C

Rev Tm 61.8°C

GC 60%

Product 117 bp

Spans 2 introns

NM_001044983 · chr18

115 Specific

FWD 5' AGACCAACTCTCGGGTTCTG 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.0°C

Rev Tm 61.8°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001044983 · chr18_KZ115554v1_alt

115 Specific

FWD 5' AGACCAACTCTCGGGTTCTG 3'

REV 5' GTCCGGTGCACAGATGTGTA 3'

Fwd Tm 61.0°C

Rev Tm 61.8°C

GC 55%

Product 149 bp

Spans 2 introns