akt1 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001281801 · chr17

118 Specific

FWD 5' GGCCCAATGTCAGCTGATGA 3'

REV 5' GTCTGGATGGCTTTGGTCCA 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001281801 · chr17

118 Specific

FWD 5' AGTGCTGCAGAACTCCAGAC 3'

REV 5' ACACGCTCTCGAGACAAGTG 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001281801 · chr17

118 Specific

FWD 5' AGTGCTGCAGAACTCCAGAC 3'

REV 5' CACACGCTCTCGAGACAAGT 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 133 bp

Spans 2 introns

NM_001281801 · chr17

118 Specific

FWD 5' AGTGCTGCAGAACTCCAGAC 3'

REV 5' ACACACGCTCTCGAGACAAG 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001281801 · chr17

118 Specific

FWD 5' AGTGCTGCAGAACTCCAGAC 3'

REV 5' GAACACACGCTCTCGAGACA 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001281801 · chr17

118 Specific

FWD 5' GAGTGCTGCAGAACTCCAGA 3'

REV 5' GAACACACGCTCTCGAGACA 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001281801 · chr17

118 Specific

FWD 5' GAGTGCTGCAGAACTCCAGA 3'

REV 5' ACACGCTCTCGAGACAAGTG 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 133 bp

Spans 2 introns

NM_001281801 · chr17

118 Specific

FWD 5' GAGTGCTGCAGAACTCCAGA 3'

REV 5' CACACGCTCTCGAGACAAGT 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001281801 · chr17

118 Specific

FWD 5' GAGTGCTGCAGAACTCCAGA 3'

REV 5' ACACACGCTCTCGAGACAAG 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 135 bp

Spans 2 introns

NM_001281801 · chr17

118 Specific

FWD 5' GAGAACCGAGTGCTGCAGAA 3'

REV 5' ACACGCTCTCGAGACAAGTG 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 55%

Product 140 bp

Spans 2 introns