akt2 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_198146 · chr18

119 Specific

FWD 5' CGCCATGAAGATCCTACGCA 3'

REV 5' AACACAGTCGATCTCGCGTC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 146 bp

Spans 2 introns

NM_198146 · chr18

119 Specific

FWD 5' ACGCCATGAAGATCCTACGC 3'

REV 5' AACACAGTCGATCTCGCGTC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 147 bp

Spans 2 introns

NM_198146 · chr18

119 Specific

FWD 5' GTTGCAGAACACACGGCATC 3'

REV 5' CCGTAAACACACGCTCTCGA 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 137 bp

Spans 2 introns

NM_198146 · chr18

119 Specific

FWD 5' GTTGCAGAACACACGGCATC 3'

REV 5' TCCGTAAACACACGCTCTCG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 138 bp

Spans 2 introns

NM_198146 · chr18

119 Specific

FWD 5' GTTGCAGAACACACGGCATC 3'

REV 5' GTCCTCCGTAAACACACGCT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 142 bp

Spans 2 introns

NM_198146 · chr18

119 Specific

FWD 5' GAAGCTCGTTCCACCCTTCA 3'

REV 5' AGTGTGTGCGTGTATCTGGG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 149 bp

Spans 2 introns

NM_198146 · chr18

119 Specific

FWD 5' AGCTCGTTCCACCCTTCAAG 3'

REV 5' AGTGTGTGCGTGTATCTGGG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 147 bp

Spans 2 introns

NM_198146 · chr18

119 Specific

FWD 5' GAAGCTCGTTCCACCCTTCA 3'

REV 5' TGTGTGCGTGTATCTGGGTC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 147 bp

Spans 2 introns

NM_198146 · chr18

119 Specific

FWD 5' AGCTCGTTCCACCCTTCAAG 3'

REV 5' TGTGTGCGTGTATCTGGGTC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 145 bp

Spans 2 introns

NM_198146 · chr18

119 Specific

FWD 5' AGCTCGTTCCACCCTTCAAG 3'

REV 5' GTGTGTGCGTGTATCTGGGT 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 2 introns