alad 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001017645 · chr5

119 Specific

FWD 5' GTTGCTTTGGCATACGCTCG 3'

REV 5' AGCACTGTAGCTCAGCACTG 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001017645 · chr5

119 Specific

FWD 5' GTTGCTTTGGCATACGCTCG 3'

REV 5' GCACTGTAGCTCAGCACTGA 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001017645 · chr5

118 Specific

FWD 5' GTTCAGAGATGCTGCCCAGT 3'

REV 5' CCATCAGCATGTCAGCACCT 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 131 bp

Spans 2 introns

NM_001017645 · chr5

117 Specific

FWD 5' GTTCAGAGATGCTGCCCAGT 3'

REV 5' ACCATCAGCATGTCAGCACC 3'

Fwd Tm 61.9°C

Rev Tm 62.5°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001017645 · chr5

117 Specific

FWD 5' TCAGAGATGCTGCCCAGTCT 3'

REV 5' ACCATCAGCATGTCAGCACC 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 130 bp

Spans 2 introns

NM_001017645 · chr5

117 Specific

FWD 5' TTCAGAGATGCTGCCCAGTC 3'

REV 5' CCATCAGCATGTCAGCACCT 3'

Fwd Tm 61.6°C

Rev Tm 62.3°C

GC 55%

Product 130 bp

Spans 2 introns

NM_001017645 · chr5

117 Specific

FWD 5' TCAGAGATGCTGCCCAGTCT 3'

REV 5' CCATCAGCATGTCAGCACCT 3'

Fwd Tm 62.7°C

Rev Tm 62.3°C

GC 55%

Product 129 bp

Spans 2 introns

NM_001017645 · chr5

117 Specific

FWD 5' AGGTGTCAGTGCTGAGCTAC 3'

REV 5' GGCACCAGGAGGTAACTGAT 3'

Fwd Tm 61.2°C

Rev Tm 61.2°C

GC 55%

Product 122 bp

Spans 2 introns

NM_001017645 · chr5

116 Specific

FWD 5' GCAACAAGGTGTCAGTGCTG 3'

REV 5' AAAGCAGGCTTAGACTGGGC 3'

Fwd Tm 61.5°C

Rev Tm 62.2°C

GC 55%

Product 91 bp

Spans 2 introns

NM_001017645 · chr5

116 Specific

FWD 5' TGGACCGTTCAGAGATGCTG 3'

REV 5' CCATCAGCATGTCAGCACCT 3'

Fwd Tm 61.5°C

Rev Tm 62.3°C

GC 55%

Product 137 bp

Spans 2 introns