alas2 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_131682 · chr8_KZ115258v1_alt

110 Specific

FWD 5' AGAAAGATGGAGCGCTGGTC 3'

REV 5' CCCACTGTTTCTGATGCCCT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 1 intron

NM_131682 · chr8_KZ115258v1_alt

110 Specific

FWD 5' GAAAGATGGAGCGCTGGTCT 3'

REV 5' CCCACTGTTTCTGATGCCCT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 1 intron

NM_131682 · chr8

110 Specific

FWD 5' AGAAAGATGGAGCGCTGGTC 3'

REV 5' CCCACTGTTTCTGATGCCCT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 1 intron

NM_131682 · chr8

110 Specific

FWD 5' GAAAGATGGAGCGCTGGTCT 3'

REV 5' CCCACTGTTTCTGATGCCCT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 1 intron

NM_131682 · chr8_KZ115258v1_alt

109 Specific

FWD 5' CTCAGTGCAGGTCAACAGCT 3'

REV 5' ATCTGGCGGACAATGATGGG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 145 bp

Spans 1 intron

NM_131682 · chr8

109 Specific

FWD 5' CTCAGTGCAGGTCAACAGCT 3'

REV 5' ATCTGGCGGACAATGATGGG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 145 bp

Spans 1 intron

NM_131682 · chr8_KZ115258v1_alt

109 Specific

FWD 5' CCACAAGTGCAGGAGGATGT 3'

REV 5' AGGTGTGTAGGACCAGTGGA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 113 bp

Spans 1 intron

NM_131682 · chr8

109 Specific

FWD 5' CCACAAGTGCAGGAGGATGT 3'

REV 5' AGGTGTGTAGGACCAGTGGA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 113 bp

Spans 1 intron

NM_131682 · chr8_KZ115258v1_alt

109 Specific

FWD 5' ATGATGCCAGCCATCTGGAG 3'

REV 5' ACACAGCTCTTCAAGAGGGC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 119 bp

Spans 1 intron

NM_131682 · chr8

109 Specific

FWD 5' ATGATGCCAGCCATCTGGAG 3'

REV 5' ACACAGCTCTTCAAGAGGGC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 119 bp

Spans 1 intron

NM_131682 · chr8_KZ115258v1_alt

109 Specific

FWD 5' ATGATGCCAGCCATCTGGAG 3'

REV 5' CACAGCTCTTCAAGAGGGCA 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 118 bp

Spans 1 intron

NM_131682 · chr8

109 Specific

FWD 5' ATGATGCCAGCCATCTGGAG 3'

REV 5' CACAGCTCTTCAAGAGGGCA 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 118 bp

Spans 1 intron

NM_131682 · chr8_KZ115258v1_alt

109 Specific

FWD 5' CTCAGTGCAGGTCAACAGCT 3'

REV 5' ATCGGTCAGCCAAGTTGAGG 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 123 bp

Spans 1 intron

NM_131682 · chr8

109 Specific

FWD 5' CTCAGTGCAGGTCAACAGCT 3'

REV 5' ATCGGTCAGCCAAGTTGAGG 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 123 bp

Spans 1 intron

NM_131682 · chr8_KZ115258v1_alt

109 Specific

FWD 5' CACCAGAAAGATGGAGCGCT 3'

REV 5' CCCACTGTTTCTGATGCCCT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 150 bp

Spans 1 intron

NM_131682 · chr8_KZ115258v1_alt

109 Specific

FWD 5' ACCAGAAAGATGGAGCGCTG 3'

REV 5' CCCACTGTTTCTGATGCCCT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 1 intron

NM_131682 · chr8_KZ115258v1_alt

109 Specific

FWD 5' CAGAAAGATGGAGCGCTGGT 3'

REV 5' CCCACTGTTTCTGATGCCCT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 147 bp

Spans 1 intron

NM_131682 · chr8

109 Specific

FWD 5' CACCAGAAAGATGGAGCGCT 3'

REV 5' CCCACTGTTTCTGATGCCCT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 150 bp

Spans 1 intron

NM_131682 · chr8

109 Specific

FWD 5' ACCAGAAAGATGGAGCGCTG 3'

REV 5' CCCACTGTTTCTGATGCCCT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 1 intron

NM_131682 · chr8

109 Specific

FWD 5' CAGAAAGATGGAGCGCTGGT 3'

REV 5' CCCACTGTTTCTGATGCCCT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 147 bp

Spans 1 intron