alg12 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001098749 · chr4_KZ115095v1_alt

109 Specific

FWD 5' GTCACTTGTGGCCAGTCTGT 3'

REV 5' GCCAAGAGGTGAATGCCAGA 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 125 bp

Spans 1 intron

NM_001098749 · chr4

109 Specific

FWD 5' GTCACTTGTGGCCAGTCTGT 3'

REV 5' GCCAAGAGGTGAATGCCAGA 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 125 bp

Spans 1 intron

NM_001098749 · chr4_KZ115095v1_alt

109 Specific

FWD 5' GTCACTTGTGGCCAGTCTGT 3'

REV 5' AGCCAAGAGGTGAATGCCAG 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001098749 · chr4

109 Specific

FWD 5' GTCACTTGTGGCCAGTCTGT 3'

REV 5' AGCCAAGAGGTGAATGCCAG 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001098749 · chr4_KZ115095v1_alt

109 Specific

FWD 5' CGAAGGCAGTTTGGGTCACT 3'

REV 5' AGCCAAGAGGTGAATGCCAG 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 140 bp

Spans 1 intron

NM_001098749 · chr4

109 Specific

FWD 5' CGAAGGCAGTTTGGGTCACT 3'

REV 5' AGCCAAGAGGTGAATGCCAG 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 140 bp

Spans 1 intron

NM_001098749 · chr4_KZ115095v1_alt

109 Specific

FWD 5' CGAAGGCAGTTTGGGTCACT 3'

REV 5' GCCAAGAGGTGAATGCCAGA 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 139 bp

Spans 1 intron

NM_001098749 · chr4

109 Specific

FWD 5' CGAAGGCAGTTTGGGTCACT 3'

REV 5' GCCAAGAGGTGAATGCCAGA 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 139 bp

Spans 1 intron

NM_001098749 · chr4_KZ115095v1_alt

108 Specific

FWD 5' GTGGCCAGTCTGTTCTGCTT 3'

REV 5' AGCCAAGAGGTGAATGCCAG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 119 bp

Spans 1 intron

NM_001098749 · chr4

108 Specific

FWD 5' GTGGCCAGTCTGTTCTGCTT 3'

REV 5' AGCCAAGAGGTGAATGCCAG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 119 bp

Spans 1 intron

NM_001098749 · chr4_KZ115095v1_alt

108 Specific

FWD 5' GTGGCCAGTCTGTTCTGCTT 3'

REV 5' GCCAAGAGGTGAATGCCAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001098749 · chr4

108 Specific

FWD 5' GTGGCCAGTCTGTTCTGCTT 3'

REV 5' GCCAAGAGGTGAATGCCAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001098749 · chr4_KZ115095v1_alt

108 Specific

FWD 5' AAGTCCGAAGGCAGTTTGGG 3'

REV 5' AGCCAAGAGGTGAATGCCAG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001098749 · chr4

108 Specific

FWD 5' AAGTCCGAAGGCAGTTTGGG 3'

REV 5' AGCCAAGAGGTGAATGCCAG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001098749 · chr4_KZ115095v1_alt

108 Specific

FWD 5' AAGTCCGAAGGCAGTTTGGG 3'

REV 5' GCCAAGAGGTGAATGCCAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 144 bp

Spans 1 intron

NM_001098749 · chr4

108 Specific

FWD 5' AAGTCCGAAGGCAGTTTGGG 3'

REV 5' GCCAAGAGGTGAATGCCAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 144 bp

Spans 1 intron

NM_001098749 · chr4_KZ115095v1_alt

108 Specific

FWD 5' GTGGCCAGTCTGTTCTGCTT 3'

REV 5' TGAATGCGCCATGTCTCTGG 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001098749 · chr4

108 Specific

FWD 5' GTGGCCAGTCTGTTCTGCTT 3'

REV 5' TGAATGCGCCATGTCTCTGG 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001098749 · chr4_KZ115095v1_alt

108 Specific

FWD 5' AAGTCCGAAGGCAGTTTGGG 3'

REV 5' CAGCCAAGAGGTGAATGCCA 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001098749 · chr4

108 Specific

FWD 5' AAGTCCGAAGGCAGTTTGGG 3'

REV 5' CAGCCAAGAGGTGAATGCCA 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 146 bp

Spans 1 intron