alg13 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001031838 · chr21

120 Specific

FWD 5' GCAGCGACCTTCCAAGAGAT 3'

REV 5' GTGCACCATAGAGTGCCAGT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 95 bp

Spans 2 introns

NM_001031838 · chr21

119 Specific

FWD 5' ACAGCCATGCAGAGCAGTAC 3'

REV 5' AAATCAGACCGGGAGGAGGA 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 117 bp

Spans 2 introns

NM_001031838 · chr21

119 Specific

FWD 5' GGAGTGCTGGTGGTGATCAA 3'

REV 5' CGCACGAGAACTCCCTTCTT 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 143 bp

Spans 3 introns

NM_001031838 · chr21

119 Specific

FWD 5' GCTGGTGGTGATCAAGGTGA 3'

REV 5' CGCACGAGAACTCCCTTCTT 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 138 bp

Spans 3 introns

NM_001031838 · chr21

119 Specific

FWD 5' AGTGCTGGTGGTGATCAAGG 3'

REV 5' CGCACGAGAACTCCCTTCTT 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 141 bp

Spans 3 introns

NM_001031838 · chr21

118 Specific

FWD 5' ATCTACTCTGCCGCAGAAGC 3'

REV 5' CGCACGAGAACTCCCTTCTT 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 102 bp

Spans 2 introns

NM_001031838 · chr21

118 Specific

FWD 5' ATCTACTCTGCCGCAGAAGC 3'

REV 5' TGTAGGTAGCGACACCCTCT 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 133 bp

Spans 2 introns

NM_001031838 · chr21

118 Specific

FWD 5' GCAGCGACCTTCCAAGAGAT 3'

REV 5' AGTGCACCATAGAGTGCCAG 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 96 bp

Spans 2 introns

NM_001031838 · chr21

118 Specific

FWD 5' ATCTACTCTGCCGCAGAAGC 3'

REV 5' ACTGTAGGTAGCGACACCCT 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 2 introns

NM_001031838 · chr21

118 Specific

FWD 5' TGCTGGTGGTGATCAAGGTG 3'

REV 5' CGCACGAGAACTCCCTTCTT 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 139 bp

Spans 3 introns