alg3 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001020696 · chr2

111 Specific

FWD 5' GGTCTGGGAGCAGTATTTGG 3'

REV 5' GCAGAGATCTGCTGAAGCAC 3'

Fwd Tm 59.9°C

Rev Tm 60.6°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001020696 · chr2

110 Specific

FWD 5' GGTCTGGGAGCAGTATTTGG 3'

REV 5' GATCTGCTGAAGCACATGCC 3'

Fwd Tm 59.9°C

Rev Tm 61.2°C

GC 55%

Product 133 bp

Spans 2 introns

NM_001020696 · chr2

109 Specific

FWD 5' AGGAGTGAAGAAGCTGGCAC 3'

REV 5' ATGATGAGGTGGCAGACGTG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 123 bp

Spans 1 intron

NM_001020696 · chr2

109 Specific

FWD 5' AATCTCCTGCTCGTGATGCG 3'

REV 5' GGCCACCGGATCATTGAAGA 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 132 bp

Spans 1 intron

NM_001020696 · chr2

109 Specific

FWD 5' AATCTCCTGCTCGTGATGCG 3'

REV 5' CATCATGGCCACCGGATCAT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 138 bp

Spans 1 intron

NM_001020696 · chr2

109 Specific

FWD 5' AATCTCTTTCTGGACGGCCG 3'

REV 5' ACAGCAGGAGGAAGAGGAGA 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 112 bp

Spans 1 intron

NM_001020696 · chr2

109 Specific

FWD 5' GGAGTGAAGAAGCTGGCACA 3'

REV 5' ATGATGAGGTGGCAGACGTG 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 122 bp

Spans 1 intron

NM_001020696 · chr2

109 Specific

FWD 5' AATCTCCTGCTCGTGATGCG 3'

REV 5' TGGCCACCGGATCATTGAAG 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 133 bp

Spans 1 intron

NM_001020696 · chr2

109 Specific

FWD 5' GGTCTGGGAGCAGTATTTGG 3'

REV 5' AGATCTGCTGAAGCACATGC 3'

Fwd Tm 59.9°C

Rev Tm 60.2°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001020696 · chr2

109 Specific

FWD 5' CTTCAATGATCCGGTGGCCA 3'

REV 5' AGAGGAGACCAGGAGCGAAA 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 143 bp

Spans 1 intron