alg9 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_200625 · chr5

118 Specific

FWD 5' TGCTCTTTCTGGTCCCTGTG 3'

REV 5' AGTAATGCCAGGGCTCTGTG 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 138 bp

Spans 2 introns

NM_200625 · chr5

118 Specific

FWD 5' GGCATTGCTGCTCTTTCTGG 3'

REV 5' AGTAATGCCAGGGCTCTGTG 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 146 bp

Spans 2 introns

NM_200625 · chr5

118 Specific

FWD 5' TTGCTGCTCTTTCTGGTCCC 3'

REV 5' AGTAATGCCAGGGCTCTGTG 3'

Fwd Tm 62.2°C

Rev Tm 61.7°C

GC 55%

Product 142 bp

Spans 2 introns

NM_200625 · chr5

117 Specific

FWD 5' CTGCTCTTTCTGGTCCCTGT 3'

REV 5' AGTAATGCCAGGGCTCTGTG 3'

Fwd Tm 61.3°C

Rev Tm 61.7°C

GC 55%

Product 139 bp

Spans 2 introns

NM_200625 · chr5

117 Specific

FWD 5' CTTCCTGCCTGCTTTCATGC 3'

REV 5' CGTCCCACATGTAGGCCAAA 3'

Fwd Tm 61.5°C

Rev Tm 62.2°C

GC 55%

Product 149 bp

Spans 2 introns

NM_200625 · chr5

117 Specific

FWD 5' TGCTTGGGATACCCATTGCC 3'

REV 5' AGGAGCAATCACCAGCTTGC 3'

Fwd Tm 62.4°C

Rev Tm 62.8°C

GC 55%

Product 149 bp

Spans 2 introns

NM_200625 · chr5

116 Specific

FWD 5' GCTTGGGATACCCATTGCCT 3'

REV 5' AGGAGCAATCACCAGCTTGC 3'

Fwd Tm 62.2°C

Rev Tm 62.8°C

GC 55%

Product 148 bp

Spans 2 introns

NM_200625 · chr5

116 Specific

FWD 5' GCTTGGGATACCCATTGCCT 3'

REV 5' AGCAATCACCAGCTTGCCAT 3'

Fwd Tm 62.2°C

Rev Tm 62.4°C

GC 55%

Product 145 bp

Spans 2 introns

NM_200625 · chr5

116 Specific

FWD 5' TGCTTGGGATACCCATTGCC 3'

REV 5' AGCAATCACCAGCTTGCCAT 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 146 bp

Spans 2 introns

NM_200625 · chr5

115 Specific

FWD 5' CTCTTTCTGGTCCCTGTGGT 3'

REV 5' AGTAATGCCAGGGCTCTGTG 3'

Fwd Tm 61.0°C

Rev Tm 61.7°C

GC 55%

Product 136 bp

Spans 2 introns