alpi.2 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001025188 · chr22

117 Specific

FWD 5' ATGGCATGGGAATCAGCACC 3'

REV 5' GGCATCTGCTGGTCCACATT 3'

Fwd Tm 62.7°C

Rev Tm 62.6°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001025188 · chr22

116 Specific

FWD 5' CGGAGATGGCATGGGAATCA 3'

REV 5' GGCATCTGCTGGTCCACATT 3'

Fwd Tm 61.8°C

Rev Tm 62.6°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001025188 · chr22

116 Specific

FWD 5' GGGAGCCGGAAAGACAAGAG 3'

REV 5' CCTGGTGTCCTTTGGCTCAA 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 60%

Product 150 bp

Spans 2 introns

NM_001025188 · chr22

116 Specific

FWD 5' GGAGCCGGAAAGACAAGAGA 3'

REV 5' CCTGGTGTCCTTTGGCTCAA 3'

Fwd Tm 61.3°C

Rev Tm 62.1°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001025188 · chr22

115 Specific

FWD 5' CCGTTGTTCCTGAGGAGGAG 3'

REV 5' TCACGGTGCTGATTCCCATG 3'

Fwd Tm 61.8°C

Rev Tm 62.2°C

GC 60%

Product 150 bp

Spans 2 introns

NM_001025188 · chr22

113 Specific

FWD 5' GATGGCATGGGAATCAGCAC 3'

REV 5' GGCATCTGCTGGTCCACATT 3'

Fwd Tm 61.1°C

Rev Tm 62.6°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001025188 · chr22

112 Specific

FWD 5' ATGGCATGGGAATCAGCACC 3'

REV 5' AGGCATCTGCTGGTCCACAT 3'

Fwd Tm 62.7°C

Rev Tm 63.6°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001025188 · chr22

112 Specific

FWD 5' CGTTGTTCCTGAGGAGGAGA 3'

REV 5' TCACGGTGCTGATTCCCATG 3'

Fwd Tm 60.7°C

Rev Tm 62.2°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001025188 · chr22

112 Specific

FWD 5' CGTTGTTCCTGAGGAGGAGA 3'

REV 5' GTCACGGTGCTGATTCCCAT 3'

Fwd Tm 60.7°C

Rev Tm 62.2°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001025188 · chr22

112 Specific

FWD 5' CCTGAGGAGGAGAAGAACCC 3'

REV 5' TCACGGTGCTGATTCCCATG 3'

Fwd Tm 61.1°C

Rev Tm 62.2°C

GC 60%

Product 142 bp

Spans 2 introns