ambp 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_201118 · chr10

120 Specific

FWD 5' ACCAGAGCCACAGATTCAGC 3'

REV 5' ACATCCCGAAACATGGACCC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 140 bp

Spans 2 introns

NM_201118 · chr10

120 Specific

FWD 5' CCACACCAGAGCCACAGATT 3'

REV 5' ACATCCCGAAACATGGACCC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 144 bp

Spans 2 introns

NM_201118 · chr10

119 Specific

FWD 5' CCACACCAGAGCCACAGATT 3'

REV 5' CTGGCTCCGACTTACATGCA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 120 bp

Spans 2 introns

NM_201118 · chr10

119 Specific

FWD 5' ACCAGAGCCACAGATTCAGC 3'

REV 5' CTGGCTCCGACTTACATGCA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 116 bp

Spans 2 introns

NM_201118 · chr10

119 Specific

FWD 5' ACTCTGGTGGCAGAACAAGG 3'

REV 5' ACAACCACATTCCTCCTCGC 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 128 bp

Spans 2 introns

NM_201118 · chr10

118 Specific

FWD 5' ACCAGAGCCACAGATTCAGC 3'

REV 5' CCCGAAACATGGACCCGAAT 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 136 bp

Spans 2 introns

NM_201118 · chr10

118 Specific

FWD 5' CCACACCAGAGCCACAGATT 3'

REV 5' CCCGAAACATGGACCCGAAT 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 140 bp

Spans 2 introns

NM_201118 · chr10

118 Specific

FWD 5' ACCAGAGCCACAGATTCAGC 3'

REV 5' AACATGGACCCGAATCTGGC 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 131 bp

Spans 2 introns

NM_201118 · chr10

118 Specific

FWD 5' CCACACCAGAGCCACAGATT 3'

REV 5' AACATGGACCCGAATCTGGC 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 135 bp

Spans 2 introns

NM_201118 · chr10

118 Specific

FWD 5' ACTCTGGTGGCAGAACAAGG 3'

REV 5' GGCACAACCACATTCCTCCT 3'

Fwd Tm 61.8°C

Rev Tm 62.2°C

GC 55%

Product 131 bp

Spans 2 introns