amer1 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001346277 · chr5

110 Specific

FWD 5' CTGCACTCTGTGACGGACAA 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 95 bp

Spans 1 intron

NM_001346277 · chr5

110 Specific

FWD 5' TGCACTCTGTGACGGACAAG 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 94 bp

Spans 1 intron

NM_001346277 · chr5_KZ115109v1_alt

110 Specific

FWD 5' CTGCACTCTGTGACGGACAA 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 95 bp

Spans 1 intron

NM_001346277 · chr5_KZ115109v1_alt

110 Specific

FWD 5' TGCACTCTGTGACGGACAAG 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 94 bp

Spans 1 intron

NM_001346277 · chr5

109 Specific

FWD 5' CTGCGTGAGGAGAAAGCTCA 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001346277 · chr5

109 Specific

FWD 5' ATCACTGCACTCTGTGACGG 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001346277 · chr5_KZ115109v1_alt

109 Specific

FWD 5' CTGCGTGAGGAGAAAGCTCA 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001346277 · chr5_KZ115109v1_alt

109 Specific

FWD 5' ATCACTGCACTCTGTGACGG 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001346277 · chr5

108 Specific

FWD 5' GCACTCTGTGACGGACAAGA 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 93 bp

Spans 1 intron

NM_001346277 · chr5_KZ115109v1_alt

108 Specific

FWD 5' GCACTCTGTGACGGACAAGA 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 93 bp

Spans 1 intron

NM_001346277 · chr5

106 Specific

FWD 5' CTGCGTGAGGAGAAAGCTCA 3'

REV 5' GGCTGGAACAGATGAGGACT 3'

Fwd Tm 61.8°C

Rev Tm 61.1°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001346277 · chr5

106 Specific

FWD 5' CTGCGTGAGGAGAAAGCTCA 3'

REV 5' AGGCTGGAACAGATGAGGAC 3'

Fwd Tm 61.8°C

Rev Tm 61.1°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001346277 · chr5_KZ115109v1_alt

106 Specific

FWD 5' CTGCGTGAGGAGAAAGCTCA 3'

REV 5' GGCTGGAACAGATGAGGACT 3'

Fwd Tm 61.8°C

Rev Tm 61.1°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001346277 · chr5_KZ115109v1_alt

106 Specific

FWD 5' CTGCGTGAGGAGAAAGCTCA 3'

REV 5' AGGCTGGAACAGATGAGGAC 3'

Fwd Tm 61.8°C

Rev Tm 61.1°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001346277 · chr5

106 Specific

FWD 5' CTGCGTGAGGAGAAAGCTCA 3'

REV 5' CAGGCTGGAACAGATGAGGA 3'

Fwd Tm 61.8°C

Rev Tm 61.1°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001346277 · chr5_KZ115109v1_alt

106 Specific

FWD 5' CTGCGTGAGGAGAAAGCTCA 3'

REV 5' CAGGCTGGAACAGATGAGGA 3'

Fwd Tm 61.8°C

Rev Tm 61.1°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001346277 · chr5

105 Specific

FWD 5' TCACTGCACTCTGTGACGGA 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 62.9°C

Rev Tm 61.9°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001346277 · chr5_KZ115109v1_alt

105 Specific

FWD 5' TCACTGCACTCTGTGACGGA 3'

REV 5' ATACTCCACCCTGAGAGGCA 3'

Fwd Tm 62.9°C

Rev Tm 61.9°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001346277 · chr5

104 Specific

FWD 5' GCTCATCACTGCACTCTGTG 3'

REV 5' AATACTCCACCCTGAGAGGC 3'

Fwd Tm 60.5°C

Rev Tm 60.6°C

GC 55%

Product 104 bp

Spans 1 intron

NM_001346277 · chr5_KZ115109v1_alt

104 Specific

FWD 5' GCTCATCACTGCACTCTGTG 3'

REV 5' AATACTCCACCCTGAGAGGC 3'

Fwd Tm 60.5°C

Rev Tm 60.6°C

GC 55%

Product 104 bp

Spans 1 intron