amfra 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_213163 · chr25

118 Specific

FWD 5' GCATGCACACTGTCGCTTTC 3'

REV 5' TGTTCTCCCAGGTTCCCTCA 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 123 bp

Spans 2 introns

NM_213163 · chr25

118 Specific

FWD 5' TCTCGCCGGTCATATTCACG 3'

REV 5' CCAGGTTCCCTCATGGTTCA 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 55%

Product 136 bp

Spans 2 introns

NM_213163 · chr25

118 Specific

FWD 5' GCACACTGTCGCTTTCATGG 3'

REV 5' CCAGGTTCCCTCATGGTTCA 3'

Fwd Tm 61.6°C

Rev Tm 61.4°C

GC 55%

Product 112 bp

Spans 2 introns

NM_213163 · chr25

118 Specific

FWD 5' CACACTGTCGCTTTCATGGC 3'

REV 5' CCAGGTTCCCTCATGGTTCA 3'

Fwd Tm 61.6°C

Rev Tm 61.4°C

GC 55%

Product 111 bp

Spans 2 introns

NM_213163 · chr25

118 Specific

FWD 5' GCCGGTCATATTCACGGCAT 3'

REV 5' TGTTCTCCCAGGTTCCCTCA 3'

Fwd Tm 62.6°C

Rev Tm 62.4°C

GC 55%

Product 139 bp

Spans 2 introns

NM_213163 · chr25

118 Specific

FWD 5' GCAGCCGAGTGTTTGCTAGT 3'

REV 5' TGTTCTCCCAGGTTCCCTCA 3'

Fwd Tm 62.6°C

Rev Tm 62.4°C

GC 55%

Product 100 bp

Spans 2 introns

NM_213163 · chr25

118 Specific

FWD 5' GCTCTCGCCGGTCATATTCA 3'

REV 5' CCAGGTTCCCTCATGGTTCA 3'

Fwd Tm 61.6°C

Rev Tm 61.4°C

GC 55%

Product 138 bp

Spans 2 introns

NM_213163 · chr25

117 Specific

FWD 5' CACTGTCGCTTTCATGGCAG 3'

REV 5' TGGTTCAGATCCCACAGGTG 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 96 bp

Spans 2 introns

NM_213163 · chr25

117 Specific

FWD 5' CACTGTCGCTTTCATGGCAG 3'

REV 5' CCAGGTTCCCTCATGGTTCA 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 109 bp

Spans 2 introns

NM_213163 · chr25

117 Specific

FWD 5' TCTCGCCGGTCATATTCACG 3'

REV 5' TGGTTCAGATCCCACAGGTG 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 55%

Product 123 bp

Spans 2 introns