ampd3b 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_199848 · chr18

116 Specific

FWD 5' AGGAGCGTGGTCTCAATACC 3'

REV 5' TACAGGTACTGCAGCACAGG 3'

Fwd Tm 61.1°C

Rev Tm 61.3°C

GC 55%

Product 145 bp

Spans 2 introns

NM_199848 · chr18_KZ115554v1_alt

116 Specific

FWD 5' AGGAGCGTGGTCTCAATACC 3'

REV 5' TACAGGTACTGCAGCACAGG 3'

Fwd Tm 61.1°C

Rev Tm 61.3°C

GC 55%

Product 145 bp

Spans 2 introns

NM_199848 · chr18

112 Specific

FWD 5' AAGAAAGGAGCGTGGTCTCA 3'

REV 5' TACAGGTACTGCAGCACAGG 3'

Fwd Tm 60.7°C

Rev Tm 61.3°C

GC 50%

Product 150 bp

Spans 2 introns

NM_199848 · chr18

112 Specific

FWD 5' AGAAAGGAGCGTGGTCTCAA 3'

REV 5' TACAGGTACTGCAGCACAGG 3'

Fwd Tm 60.7°C

Rev Tm 61.3°C

GC 50%

Product 149 bp

Spans 2 introns

NM_199848 · chr18_KZ115554v1_alt

112 Specific

FWD 5' AAGAAAGGAGCGTGGTCTCA 3'

REV 5' TACAGGTACTGCAGCACAGG 3'

Fwd Tm 60.7°C

Rev Tm 61.3°C

GC 50%

Product 150 bp

Spans 2 introns

NM_199848 · chr18_KZ115554v1_alt

112 Specific

FWD 5' AGAAAGGAGCGTGGTCTCAA 3'

REV 5' TACAGGTACTGCAGCACAGG 3'

Fwd Tm 60.7°C

Rev Tm 61.3°C

GC 50%

Product 149 bp

Spans 2 introns

NM_199848 · chr18

111 Specific

FWD 5' AGGAGCGTGGTCTCAATACC 3'

REV 5' AGGTACTGCAGCACAGGACT 3'

Fwd Tm 61.1°C

Rev Tm 62.9°C

GC 55%

Product 142 bp

Spans 2 introns

NM_199848 · chr18_KZ115554v1_alt

111 Specific

FWD 5' AGGAGCGTGGTCTCAATACC 3'

REV 5' AGGTACTGCAGCACAGGACT 3'

Fwd Tm 61.1°C

Rev Tm 62.9°C

GC 55%

Product 142 bp

Spans 2 introns

NM_199848 · chr18

110 Specific

FWD 5' AGGAGCGTGGTCTCAATACC 3'

REV 5' CAGGTACTGCAGCACAGGAC 3'

Fwd Tm 61.1°C

Rev Tm 62.6°C

GC 55%

Product 143 bp

Spans 2 introns

NM_199848 · chr18_KZ115554v1_alt

110 Specific

FWD 5' AGGAGCGTGGTCTCAATACC 3'

REV 5' CAGGTACTGCAGCACAGGAC 3'

Fwd Tm 61.1°C

Rev Tm 62.6°C

GC 55%

Product 143 bp

Spans 2 introns

NM_199848 · chr18

110 Specific

FWD 5' AGGAGCGTGGTCTCAATACC 3'

REV 5' ACAGGTACTGCAGCACAGGA 3'

Fwd Tm 61.1°C

Rev Tm 63.1°C

GC 55%

Product 144 bp

Spans 2 introns

NM_199848 · chr18_KZ115554v1_alt

110 Specific

FWD 5' AGGAGCGTGGTCTCAATACC 3'

REV 5' ACAGGTACTGCAGCACAGGA 3'

Fwd Tm 61.1°C

Rev Tm 63.1°C

GC 55%

Product 144 bp

Spans 2 introns

NM_199848 · chr18

109 Specific

FWD 5' TGGCCTGTCTCATCAGGAGA 3'

REV 5' TACGGATCTGCGCCACATTC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 104 bp

Spans 1 intron

NM_199848 · chr18_KZ115554v1_alt

109 Specific

FWD 5' TGGCCTGTCTCATCAGGAGA 3'

REV 5' TACGGATCTGCGCCACATTC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 104 bp

Spans 1 intron

NM_199848 · chr18

109 Specific

FWD 5' TGCAACAGGCTAAGGAGTGG 3'

REV 5' GGGATTTGCAGGGACATCGA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 121 bp

Spans 1 intron

NM_199848 · chr18

109 Specific

FWD 5' GGACCGCAGCCAAGTTTCTA 3'

REV 5' AGGGATCCTCACCCTCACTT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 105 bp

Spans 1 intron

NM_199848 · chr18_KZ115554v1_alt

109 Specific

FWD 5' TGCAACAGGCTAAGGAGTGG 3'

REV 5' GGGATTTGCAGGGACATCGA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 121 bp

Spans 1 intron

NM_199848 · chr18_KZ115554v1_alt

109 Specific

FWD 5' GGACCGCAGCCAAGTTTCTA 3'

REV 5' AGGGATCCTCACCCTCACTT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 105 bp

Spans 1 intron

NM_199848 · chr18

109 Specific

FWD 5' TCCAGAGGGTCACCATCAGT 3'

REV 5' GTGATATGCCAGCCGGGAAT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 125 bp

Spans 1 intron

NM_199848 · chr18_KZ115554v1_alt

109 Specific

FWD 5' TCCAGAGGGTCACCATCAGT 3'

REV 5' GTGATATGCCAGCCGGGAAT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 125 bp

Spans 1 intron