amy2a 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_213011 · chr2

113 Specific

FWD 5' GTGACATTCTGGGATGCCAG 3'

REV 5' GGAGGTCCGATCCAGTCATT 3'

Fwd Tm 60.4°C

Rev Tm 60.9°C

GC 55%

Product 146 bp

Spans 2 introns

NM_213011 · chr2

113 Specific

FWD 5' TGTGACATTCTGGGATGCCA 3'

REV 5' GGAGGTCCGATCCAGTCATT 3'

Fwd Tm 61.2°C

Rev Tm 60.9°C

GC 50%

Product 147 bp

Spans 2 introns

NM_213011 · chr2

109 Specific

FWD 5' GCACAGATGGCGTCAGATCA 3'

REV 5' AATGAACCCTCGACTGCCAC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 127 bp

Spans 1 intron

NM_213011 · chr2

109 Specific

FWD 5' GCAACACTGGCAGTGGAAAC 3'

REV 5' TGTTCATGTAGTCGGCCACC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 132 bp

Spans 1 intron

NM_213011 · chr2

109 Specific

FWD 5' ATGGCAGGCTCCACAATCTC 3'

REV 5' TCGCTTGATGTGATGGGCTC 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 106 bp

Spans 1 intron

NM_213011 · chr2

109 Specific

FWD 5' GGCAGGCTCCACAATCTCAA 3'

REV 5' TCGCTTGATGTGATGGGCTC 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 104 bp

Spans 1 intron

NM_213011 · chr2

109 Specific

FWD 5' GAGCACAGATGGCGTCAGAT 3'

REV 5' GCTGAAGGCAATCTGGTTGC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 108 bp

Spans 1 intron

NM_213011 · chr2

109 Specific

FWD 5' GAGCACAGATGGCGTCAGAT 3'

REV 5' AATGAACCCTCGACTGCCAC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 129 bp

Spans 1 intron

NM_213011 · chr2

109 Specific

FWD 5' AGCACAGATGGCGTCAGATC 3'

REV 5' GCTGAAGGCAATCTGGTTGC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 107 bp

Spans 1 intron

NM_213011 · chr2

109 Specific

FWD 5' AGCACAGATGGCGTCAGATC 3'

REV 5' AATGAACCCTCGACTGCCAC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 128 bp

Spans 1 intron