anapc13 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001302786 · chr21

110 1 gene · 2 transcripts

FWD 5' ATGCCTGGAGAGAGGACAGA 3'

REV 5' AAACTCTGCAGGGCGAGATC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001302785 · chr21

110 1 gene · 2 transcripts

FWD 5' ATGCCTGGAGAGAGGACAGA 3'

REV 5' AAACTCTGCAGGGCGAGATC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001302785 · chr21

109 Specific

FWD 5' CAGCGTTTCTGCGTCGTTTG 3'

REV 5' TGTCCTCTCTCCAGGCATCA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 121 bp

Spans 1 intron

NM_001302786 · chr21

109 1 gene · 2 transcripts

FWD 5' TGATGCCTGGAGAGAGGACA 3'

REV 5' CAAACTCTGCAGGGCGAGAT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 148 bp

Spans 1 intron

NM_001302785 · chr21

109 1 gene · 2 transcripts

FWD 5' TGATGCCTGGAGAGAGGACA 3'

REV 5' CAAACTCTGCAGGGCGAGAT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 148 bp

Spans 1 intron

NM_001302785 · chr21

109 Specific

FWD 5' CAGCGTTTCTGCGTCGTTTG 3'

REV 5' CACCATTGTCCTGCTCAGCT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 187 bp

Spans 2 introns

NM_001302786 · chr21

109 1 gene · 2 transcripts

FWD 5' TGATGCCTGGAGAGAGGACA 3'

REV 5' AAACTCTGCAGGGCGAGATC 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001302785 · chr21

109 1 gene · 2 transcripts

FWD 5' TGATGCCTGGAGAGAGGACA 3'

REV 5' AAACTCTGCAGGGCGAGATC 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001302785 · chr21

109 Specific

FWD 5' CAGCGTTTCTGCGTCGTTTG 3'

REV 5' TCTGTCCTCTCTCCAGGCAT 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 123 bp

Spans 1 intron

NM_001302786 · chr21

109 1 gene · 2 transcripts

FWD 5' ATGCCTGGAGAGAGGACAGA 3'

REV 5' CAAACTCTGCAGGGCGAGAT 3'

Fwd Tm 61.9°C

Rev Tm 62.2°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001302785 · chr21

109 1 gene · 2 transcripts

FWD 5' ATGCCTGGAGAGAGGACAGA 3'

REV 5' CAAACTCTGCAGGGCGAGAT 3'

Fwd Tm 61.9°C

Rev Tm 62.2°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001302785 · chr21

109 Specific

FWD 5' CAGCGTTTCTGCGTCGTTTG 3'

REV 5' TTGTCCTGCTCAGCTTCAGG 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 182 bp

Spans 2 introns

NM_001302786 · chr21

108 1 gene · 2 transcripts

FWD 5' TGCCATACGAGGATGTGACG 3'

REV 5' AAACTCTGCAGGGCGAGATC 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 124 bp

Spans 1 intron

NM_001302785 · chr21

108 1 gene · 2 transcripts

FWD 5' TGCCATACGAGGATGTGACG 3'

REV 5' AAACTCTGCAGGGCGAGATC 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 124 bp

Spans 1 intron

NM_001302786 · chr21

108 Specific

FWD 5' ACACAGCGAATCATGGACAG 3'

REV 5' CCATTGTCCTGCTCAGCTTC 3'

Fwd Tm 59.8°C

Rev Tm 60.6°C

GC 50%

Product 143 bp

Spans 2 introns

NM_001302786 · chr21

108 Specific

FWD 5' CACAGCGAATCATGGACAGT 3'

REV 5' CCATTGTCCTGCTCAGCTTC 3'

Fwd Tm 59.8°C

Rev Tm 60.6°C

GC 50%

Product 142 bp

Spans 2 introns

NM_001302786 · chr21

108 Specific

FWD 5' ACAGCGAATCATGGACAGTG 3'

REV 5' CCATTGTCCTGCTCAGCTTC 3'

Fwd Tm 59.8°C

Rev Tm 60.6°C

GC 50%

Product 141 bp

Spans 2 introns

NM_001302786 · chr21

108 1 gene · 2 transcripts

FWD 5' ATGCCTGGAGAGAGGACAGA 3'

REV 5' CTCTGTCGACCCACCATTGT 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 55%

Product 95 bp

Spans 1 intron

NM_001302786 · chr21

108 1 gene · 2 transcripts

FWD 5' ATGCCTGGAGAGAGGACAGA 3'

REV 5' ACTCTGTCGACCCACCATTG 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 55%

Product 96 bp

Spans 1 intron

NM_001302785 · chr21

108 1 gene · 2 transcripts

FWD 5' ATGCCTGGAGAGAGGACAGA 3'

REV 5' CTCTGTCGACCCACCATTGT 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 55%

Product 95 bp

Spans 1 intron