ankef1a 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001025543 · chr20

110 Specific

FWD 5' AAGGCGTGCTCTTCTACTGC 3'

REV 5' TCCTGAGCTTGTTCACAGGC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001025543 · chr20

110 Specific

FWD 5' TGCACAGCACTCAGATCACC 3'

REV 5' ACTGAAACGTTGCCTCCTCC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 134 bp

Spans 1 intron

NM_001025543 · chr20

110 Specific

FWD 5' GCACAGCACTCAGATCACCA 3'

REV 5' ACTGAAACGTTGCCTCCTCC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 133 bp

Spans 1 intron

NM_001025543 · chr20

110 Specific

FWD 5' TCGTGCACAGCACTCAGATC 3'

REV 5' ACTGAAACGTTGCCTCCTCC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 137 bp

Spans 1 intron

NM_001025543 · chr20

110 Specific

FWD 5' CGGCAGGCTCAACAAATTGG 3'

REV 5' ACTGAAACGTTGCCTCCTCC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 112 bp

Spans 1 intron

NM_001025543 · chr20

109 Specific

FWD 5' ACAGAGGGCGATACACCTCT 3'

REV 5' GTGCCCACAGTCCTTAGCAA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 1 intron

NM_001025543 · chr20

109 Specific

FWD 5' ACAGAGGGCGATACACCTCT 3'

REV 5' TTGTGCCCACAGTCCTTAGC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001025543 · chr20

109 Specific

FWD 5' AAGGCGTGCTCTTCTACTGC 3'

REV 5' GGAACACTGGTATGCCAGCT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 117 bp

Spans 1 intron

NM_001025543 · chr20

109 Specific

FWD 5' AAGGCGTGCTCTTCTACTGC 3'

REV 5' AGGAACACTGGTATGCCAGC 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001025543 · chr20

109 Specific

FWD 5' AAGGCGTGCTCTTCTACTGC 3'

REV 5' AGCAGGAACACTGGTATGCC 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 121 bp

Spans 1 intron