ankrd22 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_200274 · chr12

107 Specific

FWD 5' ACTCAGAGCCCATGTGTCAG 3'

REV 5' GTGTGTCTCCCGTTCCTTCA 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 114 bp

Spans 1 intron

NM_200274 · chr12

107 Specific

FWD 5' CTCGCAGCAGGTACAAAGCA 3'

REV 5' TAAGCTGCCTGACACATGGG 3'

Fwd Tm 62.6°C

Rev Tm 62.0°C

GC 55%

Product 150 bp

Spans 1 intron

NM_200274 · chr12

107 Specific

FWD 5' TCGCAGCAGGTACAAAGCAA 3'

REV 5' TAAGCTGCCTGACACATGGG 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 50%

Product 149 bp

Spans 1 intron

NM_200274 · chr12

107 Specific

FWD 5' ACTCAGAGCCCATGTGTCAG 3'

REV 5' AGGCAGCTATGATCGGTGTG 3'

Fwd Tm 61.3°C

Rev Tm 61.7°C

GC 55%

Product 129 bp

Spans 1 intron

NM_200274 · chr12

107 Specific

FWD 5' ACTCAGAGCCCATGTGTCAG 3'

REV 5' CAGGCAGCTATGATCGGTGT 3'

Fwd Tm 61.3°C

Rev Tm 61.7°C

GC 55%

Product 130 bp

Spans 1 intron

NM_200274 · chr12

107 Specific

FWD 5' ACTCAGAGCCCATGTGTCAG 3'

REV 5' ACAGGCAGCTATGATCGGTG 3'

Fwd Tm 61.3°C

Rev Tm 61.7°C

GC 55%

Product 131 bp

Spans 1 intron

NM_200274 · chr12

107 Specific

FWD 5' ACTCAGAGCCCATGTGTCAG 3'

REV 5' GGCAGCTATGATCGGTGTGT 3'

Fwd Tm 61.3°C

Rev Tm 61.9°C

GC 55%

Product 128 bp

Spans 1 intron

NM_200274 · chr12

106 Specific

FWD 5' CTCAGAGCCCATGTGTCAGG 3'

REV 5' GGCAGCTATGATCGGTGTGT 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 60%

Product 127 bp

Spans 1 intron

NM_200274 · chr12

106 Specific

FWD 5' TGCTTCAGGGACAGAGGAAG 3'

REV 5' TGTTCCTGGGATCAGCTGAG 3'

Fwd Tm 61.0°C

Rev Tm 61.1°C

GC 55%

Product 118 bp

Spans 1 intron

NM_200274 · chr12

105 Specific

FWD 5' CTCAGAGCCCATGTGTCAGG 3'

REV 5' AGGCAGCTATGATCGGTGTG 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 60%

Product 128 bp

Spans 1 intron