ankrd31 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001017911 · chr5_KZ115147v1_alt

109 Specific

FWD 5' GTCAAAGCCGCAACCAGAAC 3'

REV 5' GGGTTCAGCTGCACTACACT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 116 bp

Spans 1 intron

NM_001017911 · chr5_KZ115147v1_alt

109 Specific

FWD 5' AGGCAGTGTGCAGTGTAGTG 3'

REV 5' TCATTGGTGCTGGTCTGAGC 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 93 bp

Spans 1 intron

NM_001017911 · chr5_KZ115147v1_alt

109 Specific

FWD 5' AGGCAGTGTGCAGTGTAGTG 3'

REV 5' GCTCATTGGTGCTGGTCTGA 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 95 bp

Spans 1 intron

NM_001017911 · chr5_KZ115147v1_alt

109 Specific

FWD 5' GTCAAAGCCGCAACCAGAAC 3'

REV 5' GCTGGTCTGAGCTGATGGTT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 170 bp

Spans 2 introns

NM_001017911 · chr5_KZ115147v1_alt

109 Specific

FWD 5' AGGCAGTGTGCAGTGTAGTG 3'

REV 5' ATTGGTGCTGGTCTGAGCTG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 91 bp

Spans 1 intron

NM_001017911 · chr5_KZ115147v1_alt

109 Specific

FWD 5' AGGCAGTGTGCAGTGTAGTG 3'

REV 5' CATTGGTGCTGGTCTGAGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 92 bp

Spans 1 intron

NM_001017911 · chr5_KZ115147v1_alt

109 Specific

FWD 5' AGGCAGTGTGCAGTGTAGTG 3'

REV 5' AGCTCATTGGTGCTGGTCTG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 96 bp

Spans 1 intron

NM_001017911 · chr5_KZ115147v1_alt

109 Specific

FWD 5' GTCAAAGCCGCAACCAGAAC 3'

REV 5' CACTACACTGCACACTGCCT 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 105 bp

Spans 1 intron

NM_001017911 · chr5_KZ115147v1_alt

108 Specific

FWD 5' CCGCAACCAGAACCCTATCA 3'

REV 5' GGGTTCAGCTGCACTACACT 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 109 bp

Spans 1 intron

NM_001017911 · chr5_KZ115147v1_alt

108 Specific

FWD 5' CCGCAACCAGAACCCTATCA 3'

REV 5' GCTGGTCTGAGCTGATGGTT 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 163 bp

Spans 2 introns